|Table of Contents|

Study on the Localization and Function of Atp25 Proteinin Schizosaccharomyces Pombe(PDF)

南京师范大学学报（工程技术版）[ISSN:1006-6977/CN:61-1281/TN]

Issue:
2021年04期
Page:
80-86
Research Field:
生物工程
Publishing date:

Info

Title:
Study on the Localization and Function of Atp25 Proteinin Schizosaccharomyces Pombe
Author(s):
Zhang Yun; Huang Ying
School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
Keywords:
mitochondria; Schizosaccharomyces pombe; Atp25; respiratory chain
PACS:
Q78
DOI:
10.3969/j.issn.1672-1292.2021.04.012
Abstract:
In this paper,we investigate the localization of Atp25 protein in Schizosaccharomyces pombe and the effect of Atp25 protein deletion on mitochondrial function by fluorescence microscopy,mitochondrial extraction,gene knockout and immunoblotting. Atp25 is a nuclear genome-encoded protein. Bioinformatics predictions and fluorescence microscopy show that Atp25 protein is localized in mitochondria. The localization of Atp25 protein is further verified by immunoblotting. Phenotypic studies reveal growth and respiratory defects in strain Δatp25. The levels of mitochondrial respiratory chain complex subunit proteins(Cox1,Cox2,Cox3,Cob1,Atp6)are significantly reduced in strain Δatp25. Real-time fluorescence quantitative PCR results show no change in the mRNA levels of these complex subunits in strain Δatp25.

References:

[1] HEATH-ENGEL H M,Shore G C. Mitochondrial membrane dynamics,cristae remodelling and apoptosis[J]. Biochimica et Biophysica Acta(BBA)-Molecular Cell Research,2006,1763(5-6):549-560.
[2]BAKER B M,HAYNES C M H. Mitochondrial protein quality control during biogenesis and aging[J]. Trends in Biochemical Sciences,2011,36(5):254-261.
[3]CHAN D C. Mitochondria:dynamic organelles in disease,aging,and development[J]. Cell,2006,125(7):1241-1252.
[4]JEFFARES D C. The natural diversity and ecology of fission yeast[J]. Yeast,2018,35(3):253-260.
[5]BRAUN R J,WESTERMANN B. Mitochondrial dynamics in yeast cell death and aging[J]. Biochemical Society Transactions,2011,39(5):1520-1526.
[6]RINALDI T,DALLABONA C,FERRERO I,et al. Mitochondrial diseases and the role of the yeast models[J]. FEMS Yeast Research,2010,10(8):1006-1022.
[7]VO T D,PALSSON B O. Building the power house:recent advances in mitochondrial studies through proteomics and systems biology[J]. American Journal of Physiology Cell Physiology,2007,292(1):164-177.
[8]BHADURI S,SINGH S K,COHN W,et al. A novel chloroplast super-complex consisting of the ATP synthase and photosystem I reaction center[J]. PloS ONE,2020,15(8):e0237569.
[9]HAHN A,VONCK J,MILLS D J,et al. Structure,mechanism,and regulation of the chloroplast ATP synthase[J]. Science,2018,360(6389):eaat4318.
[10]MEHDIPOUR A R,HUMMER G. Cardiolipin puts the seal on ATP synthase[J]. Proceedings of the National Academy of Sciences,2016,113(31):8568-8570.
[11]NESCI S,TROMBETTI F,VENTRELLA V,et al. Opposite rotation directions in the synthesis and hydrolysis of ATP by the ATP Synthase:hints from a Subunit Asymmetry[J]. The Journal of Membrane Biology,2015,248(2):163-169.
[12]ARTIKA I M. Current understanding of structure,function and biogenesis of yeast mitochondrial ATP synthase[J]. Journal of Bioenergetics and Biomembranes,2019,51(5):315-328.
[13]ITOH Y,NASCHBERGER A,MORTEZAEI N,et al. Analysis of translating mitoribosome reveals functional characteristics of translation in mitochondria of fungi[J/OL]. Nature Communications,2020,11(1):1-10 [2021-04-20]. http://www.nature.com/articles/S41467-020-18830-W.
[14]LERTWATTANASAKUL N,SUPRAYOGI,MURATA M,et al. Essentiality of respiratory activity for pentose utilization in thermotolerant yeast kluyveromyces marxianus DMKU 3-1042[J]. Antonie van Leeuwenhoek,2013,103(4):933-945.
[15]ZENG X M,BARROS M H,SHULMAN T,et al. ATP25,a new nuclear gene of Saccharomyces cerevisiae required for expression and assembly of the Atp9p subunit of mitochondrial ATPase[J]. Molecular Biology of the Cell,2008,19(4):1366-1377.
[16]MALINA C,LARSSON C,NIELSEN J. Yeast mitochondria:an overview of mitochondrial biology and the potential of mitochondrial systems biology[J]. Fems Yeast Research,2018,18(5):1-17.
[17]PALERMO V,FALCONE C,MAZZONI C. Apoptosis and aging in mitochondrial morphology mutants of S.cerevisiae[J]. Folia Microbiologica,2007,52(5):479-483.
[18]谢婉秋,黄鹰. 粟酒裂殖酵母Atp10在线粒体中功能的研究[J]. 南京师大学报(自然科学版),2019,42(2):109-114.
[19]李琴,张琳琳,黄鹰. 粟酒裂殖酵母Atp4定位和功能的研究[J]. 生物技术通报,2019,35(8):59-63.
[20]WOELLHAF M W,SOMMER F,SCHRODA M,et al. Proteomic profiling of the mitochondrial ribosome identifies Atp25 as a composite mitochondrial precursor protein[J]. Molecular Biology of the Cell,2016,27(20):3031-3039.

Memo

Memo:
-
Last Update: 2021-12-15