|Table of Contents|

Optimization of Extraction of Aflatoxin from Peanuts by Response Surface Methodology and Its Application in Rapid Detection and Evaluation(PDF)

南京师范大学学报（工程技术版）[ISSN:1006-6977/CN:61-1281/TN]

Issue:
2022年03期
Page:
72-82
Research Field:
食品科学与工程
Publishing date:

Info

Title:
Optimization of Extraction of Aflatoxin from Peanuts by Response Surface Methodology and Its Application in Rapid Detection and Evaluation
Author(s):
Zhao Ping1; 2; Zhang Yingyue1; Zhou Jiahong3; Chen Fei2; 3
(1.School of Food Science and Pharmaceutical Engineering,Nanjing Normal University,Nanjing 210023,China)(2.Nanjing Engineering Research Center for Functional Components Development of Featured Biological Resources,Nanjing Normal University,Nanjing 210023,
Keywords:
response surface design; aflatoxin; quick detection kit; evaluation of validation
PACS:
TS207.3
DOI:
10.3969/j.issn.1672-1292.2022.03.010
Abstract:
The extraction process of aflatoxin(AflatoxinB1/B2/G1/G2,AFTS)from peanuts is optimized by ultrasound-assisted response surface method. The optimum extraction process is obtained as follows:65% methanol solution,ultrasonic time 15 min,ultrasonic power 206 W,and the ratio of material to liquid 1:4. Under this optimal condition,the extraction of AFTS is 18.06 μg/kg. The peanut samples are pretreated with the best technology and detected by high performance liquid chromatography(HPLC)with photochemical post-column derivation method,which is used as a reference method to evaluate the quality of the rapid detection kit. There are eight quantitative quits and two qualitative quits populared in the market to be evaluated and tested. The results show that the sensitivity of the qualitative kit is 100%, that the specificity is 98%, that the false negative rate is 0,and that the false positive rate is 2%. The accuracy and precision of the quantitative kit are high,and the linear fitting degree is more than 0.99, which can meet the detection requirements.

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Last Update: 2022-09-15