|Table of Contents|

Study on Carotenoids Production by Neurospora intermedia(PDF)

南京师范大学学报(工程技术版)[ISSN:1006-6977/CN:61-1281/TN]

Issue:
2009年01期
Page:
73-77
Research Field:
Publishing date:

Info

Title:
Study on Carotenoids Production by Neurospora intermedia
Author(s):
Tang Gang12Chen Yuru12Liu Youfen12
1.School of Life Science,Nanjing Normal University,Nanjing 210046,China;2.Jiangsu Key Lab for Biodiversity and Biotechnology,Nanjing 210046,China
Keywords:
N eurosp ora interm edia caro teno ids b iom ass aux iliary
PACS:
Q93
DOI:
-
Abstract:
The wo rk screened a strain o fN eurosp ora interm ed ia tha t y ie ld caroteno ids. The effect factors on output of caro teno ids such as culture cond itions, spec ies add itives and the illum ina tion fo rN. interm ed ia in the ferm enta tion pro cess w ere studied. The results show ed that optim um temperature o f produc ing caro teno ids w as 30e , when added 1.5 mg /L ga llic acid and 0.05 g /L phy tin respective ly into the culture med ium, the caro teno ids content of myce lium w as up to 46.44 and 54. 03 (μ g /g dry cell), the caro teno ids y ield increased by 100% and 133% ( dry ce ll) than the contro l respectively. Illum ination had an obv ious effect on prom oting ou tput o f caro te no ids, com pared w ith the contro,l the caro teno ids production increased by 73.7%. The caro teno ids content in spo res o fN. interm ed ia is as h igh as 145.5μ g /g( dry spore), sign ificantly h ighe r than that of my ce lium content. Spore production capac ity w as larger in cu ltu re so lid, so the ca rotenoids con tent o fm a terials w as 81.94μ g / g dry koji.

References:

[ 1] Br itton G, L iaaen- Jensen S, PfanderH. Caroteno ids H andbook( V II) [M ] . B ase:l Boston B irkhause rVer lag, 2004.
[ 2] M a rtaM C, Gu ille rmo SM. E ffec t o f illum ination and ch lo rophy lls on stab ility of tom ato ca ro teno ids[ J]. Food Chem istry,
2008, 107( 4): 1 365-1 370.
[ 3] Steenbo ck H, W h ite co rn V S. Ye llow corn and a probable relation betw een the fat- so lub le v itam ine and ye llow p lant pigm ents
[ J] . Science, 1919, 50: 352-353.
[ 4] Pa rk P K, K im E Y, Chu K H. Chem ical disruption of yeast cells for the iso lation of caroteno id pigm ents[ J]. Separa tion and
Pur ification Techno logy, 2007, 53( 2): 148-152.
[ 5] Packo P L, M arcel J, R ic C H De Vos, e t a.l Exploring and explo iting caro teno id accum ulation in Duna lie lla sa lina for ce l-l
factory applica tions[ J]. Trends in B io techno logy, 2008, 26( 11): 631-638.
[ 6] H u C C, L in J T, Lu F J, et a.l Dete rm ina tion of caroteno ids inDunaliella salina cultivated in Ta iw an and antiox idan t capac ity
o f the a lga l caro teno id ex tract[ J]. Food Chem istry, 2008, 109( 2) : 439-446.
[ 7] M a r?a D Q R, RosaM R V, Santiago T M, et a.l Light induction o f the caro teno id b io syn thesis pathw ay in B lakeslea trispora
[ J] . Fungal Gene tics and B io logy, 2005, 42( 2): 141-153.
[ 8] 杨先泉, 赵勤, 傅体华. 脉孢霉两对基因顺序四分子分析[ J] . 遗传, 2008, 30( 6): 801-806.
Yang X ianquan, Zhao Q in, Fu T ihua. O rdered tetrad ana ly sis o f two genes inN eurospora[ J] . H ereditas, 2008, 30( 6) : 801-
806. ( in Ch inese)
[ 9] M a rko Za loka r. Studies on b iosynthes is o f caroteno ids in N eurosp ora crassa [ J] . Arch ives of B iochem istry and B iophys ics,
1954, 50( 1): 71-80.
[ 10] 王岁楼. 红酵母产生类胡萝卜素的研究[ J]. 中国粮油学报, 2001, 16( 5) : 43-46.
W ang S L. S tudy on the conditions o f caroteno id ferm enta tion by R hodotorula [ J]. Chinese C erea ls and O ils A ssoc ia tion,
2001, 16( 5): 43-46. ( in Ch inese)
[ 11] 左亚军. 环境因素和培养条件对类胡萝卜素表达的影响[ J]. 上海化工, 2007, 32( 2): 22-26.
Zuo Y J. E ffect o f d ifferent env ironm enta l and cu ltura l stim ulants on the production of caro teno ids[ J]. Shangha iChem ica l Industry,
2007, 32( 2): 22-26. ( in Ch inese)
[ 12] 张晓蓉, 马文丽, 杨素萍. 产氢红杆菌类胡萝卜素代谢研究[ J] . 山西大学学报: 自然科学版, 2006, 29( 2): 201-204.
Zhang X R, M aW L, Yang S P. Ca rotenoid m etabo lism of hydrog en-produc ing Rhodobacter sp. [ J]. Journa l o f Shanx i Un-i
versity: Natural Science Ed ition, 2006, 29( 2): 201-204. ( in Ch inese)

Memo

Memo:
-
Last Update: 2013-04-23