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The Isolation and Purification of Dicaffeoylquinic Acidsand Chlorogenic Acid in Solidago Canadensis(PDF)

南京师范大学学报（工程技术版）[ISSN:1006-6977/CN:61-1281/TN]

Issue:
2016年01期
Page:
84-
Research Field:
生物工程
Publishing date:

Info

Title:
The Isolation and Purification of Dicaffeoylquinic Acidsand Chlorogenic Acid in Solidago Canadensis
Author(s):
Yang Fengping; Li Dongxiao; Wang Jianfen; Chen Yuru
School of Life Science，Jiangsu Engineering and Technology Research Centre for Microbiology Resource，Jiangsu Key Laboratory for Microbes andFunctional Genomic，Jiangsu Key Laboratory for Biodiversity and Biotechnology，Nanjing Normal University，Nanjing 210023，China
Keywords:
Solidago Canadensis; dicaffeoylquinic acids; chlorogenic acid; isolation and purification; macroporous resin
PACS:
TS202.3
DOI:
-
Abstract:
In this work，the natural compounds of the invasive plant Solidago Canadensis are studied，and the contents of dicaffeoylquinic acids and chlorogenic acid are analyzed. The separation and purification process are discussed. The contents of dicaffeoylquinic acids and chlorogenic acid are the highest in flower and leaf of Solidago Canadensis. The contents of dicaffeoylquinic acids and chlorogenic acid reach 1 530 mg/100 g and 700 mg/100 g in the bud of Solidago Canadensis respectively. The extraction rates of chlorogenic acid and dicaffeoylquinic acids respectively reach 90.3% and 91.6% under the optimal extraction conditions：ethanol concentration 60%，extraction temperature 50 ℃，extraction time 1 h，and solid-liquid ratio 1∶30 g/mL. The dicaffeoylquinic acids and chlorogenic acid are effectively extracted by the macroporous resin CN300 with 40%~50% and 50%~70% ethanol solution，and then having been extracted by ethyl acetate，chlorogenic acid and dicaffeoylquinic acids are obtained respectively.

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Last Update: 2016-03-20