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Study on the Antioxidant Activity of Two Kinds of Polysaccharides Compound(PDF)

南京师范大学学报(工程技术版)[ISSN:1006-6977/CN:61-1281/TN]

Issue:
2019年02期
Page:
87-
Research Field:
生物工程
Publishing date:

Info

Title:
Study on the Antioxidant Activity of Two Kinds of Polysaccharides Compound
Author(s):
Zou YuyeWang XueWang XuemeiZhu YananTao Mingxuan
School of Food Science and Pharmaceutical Engineering,Nanjing Normal University,Nanjing 210097,China
Keywords:
lentinanmomordica charantiaantioxidantcompounding ratio
PACS:
TS201.4
DOI:
10.3969/j.issn.1672-1292.2019.02.012
Abstract:
The paper studies the antioxidation of the polysaccharides of Lentinus edodes and Momordica charantia. The water extraction and alcohol precipitation method are used to obtain the crude polysaccharides of Lentinus edodes and Momordica charantia,and the protein is removed with Sevage reagent to obtain the refined polysaccharide. The scavenging activities of hydroxyl radical(·OH),superoxide anion radical(·O2-)and DPPH radical are compared between two polysaccharides and their complexes. The compounding ratio of polysaccharides of Lentinus edodes and Momordica charantia is 1:1,1:2,1:3,2:1,2:3,3:1,and 3:2. Both polysaccharides and their different proportions of complexes had good scavenging abilities for all three kinds of free radicals. When the compounding ratio is 2:1,the IC50 values of the clearance rates of hydroxy(·OH),superoxide anion(·O2-)and DPPH three radicals are 3.447 mg/mL,2.006 mg/mL,and 0.500 mg/mL respectively,and their clearance effects are better than those of the two polysaccharides and other ratios. Lentinan and momordica charantia polysaccharides has the strongest antioxidant capacity when the compounding ratio is 2:1.

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Last Update: 2019-06-30