|Table of Contents|

USP13 Inhibits Proliferation,Cloning,and Migration of ProstateCancer Cells by Upregulating STAT1(PDF)

南京师范大学学报（工程技术版）[ISSN:1006-6977/CN:61-1281/TN]

Issue:
2021年04期
Page:
72-79
Research Field:
生物工程
Publishing date:

Info

Title:
USP13 Inhibits Proliferation,Cloning,and Migration of ProstateCancer Cells by Upregulating STAT1
Author(s):
Zhang Chi1; 2; Lu Shan1; 2
(1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)(2.Jiangsu Key Laboratory for Molecular and Medical Biotechnology,Nanjing Normal University,Nanjing 210023,China)
Keywords:
prostate cancer; USP13; deubiquitination; migration; STAT1
PACS:
R737.25
DOI:
10.3969/j.issn.1672-1292.2021.04.011
Abstract:
In order to explore the role of deubiquitination enzyme USP13 in prostate cancer cells and its regulatory pathway,the eukaryotic expression plasmid of USP13 is successfully constructed via PCR,enzyme digestion,enzyme ligation and transformation into Escherichia coli. Meanwhile,CCK-8 assay,colony formation assay,wound healing assay and Transwell migration assay are performed by transfecting USP13 plasmid into PC-3 and DU145 cells. Then the effects of USP13 overexpression on mRNA experssion,protein expression and ubiquition level of signal transducer and activator of transcription 1(STAT1)are detected by reverse transcription PCR,western blotting and ubiquitination Co-IP assay,respectively. USP13 is found to inhibit the growth,cloning and migration of prostate cancer cells,which has an effect on STAT1 protein level but not on transcription level of STAT1. Overexpression of USP13 upregulates STAT1 protein level,while knockdown of USP13 decreases STAT1 protein level. Furthermore,overexpression of USP13 decreases the level of STAT1 ubiquitination,while knockdown of USP13 increases the level of STAT1 ubiquitination.

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Last Update: 2021-12-15