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Localization and Expression of Hsp60 Protein in Schizosaccharomyces pombe(PDF)

南京师范大学学报（工程技术版）[ISSN:1006-6977/CN:61-1281/TN]

Issue:
2021年04期
Page:
87-92
Research Field:
生物工程
Publishing date:

Info

Title:
Localization and Expression of Hsp60 Protein in Schizosaccharomyces pombe
Author(s):
Wang Zixuan; Shang Jinjie
School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
Keywords:
mitochondria; Hsp60; heat stress
PACS:
Q71
DOI:
10.3969/j.issn.1672-1292.2021.04.013
Abstract:
In this paper,we analyze Hsp60 protein in Schizosaccharomyces pombe by bioinformatics and protein structure. Hsp60 shares 56% or 67% homology with human and 71% or 79% homology with budding yeast,respectively. The MitoProt II software predicts that the Mitochondria targeting sequence is the first 33 amino acids of N-terminal and that there is a Chaperonin Cpn60/TCP-1 family domain. We further find that Hsp60 is localized in the mitochondrial matrix. The Hsp60 protein level of wild-type cells at 30 ℃ and 37 ℃ and different growth stages are detected. It is found that the Hsp60 protein level increases significantly at 37 ℃,but does not change at the stationary phase.

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Last Update: 2021-12-15