[1]钱媛媛,陈育如,杨凤平,等.响应面优化野艾蒿二咖啡酰奎尼酸的高效提取[J].南京师范大学学报(工程技术版),2015,15(01):073-77.
　Qian Yuanyuan,Chen Yuru,Yang Fengping,et al.Optimization Extraction of Dicaffeoylquinic Acids from Artemisialavandulaefolia DC.Using Response Surface Methodology[J].Journal of Nanjing Normal University(Engineering and Technology),2015,15(01):073-77.
点击复制

响应面优化野艾蒿二咖啡酰奎尼酸的高效提取
分享到：

南京师范大学学报（工程技术版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
15卷
期数:
2015年01期
页码:
073-77
栏目:
化学工程
出版日期:
2015-03-20

文章信息/Info

Title:
Optimization Extraction of Dicaffeoylquinic Acids from Artemisialavandulaefolia DC.Using Response Surface Methodology
作者:
钱媛媛1; 陈育如1; 杨凤平1; 柯小红1; 闫 莽1; 唐 超2
(1.南京师范大学生命科学学院,江苏省微生物资源产业化工程技术研究中心,江苏省微生物与基因组学重点实验室,江苏省生物多样性与生物技术重点实验室,江苏 南京 210023)(2.滇虹药业集团股份有限公司,云南 昆明 650106)
Author(s):
Qian Yuanyuan1; Chen Yuru1; Yang Fengping1; Ke Xiaohong1; Yan Mang1; Tang Chao2
(1.School of Life Science,Jiangsu Engineering and Technology Research Centre for Microbiology Resource,Jiangsu Key Laboratory for Biodiversityand Biotechnology,Jiangsu Key Laboratory for Microbes and Functional Genomic,Nanjing Normal University,Nanjing 2
关键词:
野艾蒿; 二咖啡酰奎尼酸; 提取; 响应面; LC/MS
Keywords:
Artemisia lavandulaefolia DC.; dicaffeoylquinic acids; extraction; response surface methodology; LC/MS
分类号:
TS202.3
文献标志码:
A
摘要:
采用响应面优化野艾蒿二咖啡酰奎尼酸的提取工艺. 考察乙醇浓度、温度、时间、料液比4个重要因素对二咖啡酰奎尼酸得率的影响. 在乙醇浓度72%、温度53 ℃、提取时间102 min、料液比1:54(g/mL)条件下,二咖啡酰奎尼酸的提取率为91%,得率达3.30%; LC/MS等方法对野艾蒿中二咖啡酰奎尼酸的定量结果表明,野艾蒿中二咖啡酰奎尼酸由3,5-diCQA、4,5-diCQA和1,5-diCQA组成,其含量分别为14.2、10.5和8.3 mg/g,因而野艾蒿是一种二咖啡酰奎尼酸含量丰富的材料.
Abstract:
Response surface methodology is applied to optimize the extraction conditions of diCQAs from Artemisia lavandulaefolia DC. The combined effects of independent variables(ethanol concentration,extraction temperature,extraction time,solid-liquid ratio)are studied and the optimal extraction conditions are as follows:ethanol concentration 72%,extraction temperature 53 ℃,extraction time 102 min,solid-liquid ratio 1:54 g/mL. Under these conditions,the yield of diCQAs is 3.30% and the extraction yield is 91%. The content of diCQAs(3,5-diCQA,4,5-diCQA,1,5-diCQA)are 14.2 mg/g,10.5 mg/g and 8.3 mg/g respectively,which are identified by LC/MS. A.lavandulaefolia DC.is the materials that rich in dicaffeoylquinic acids.

参考文献/References:

[1] Cha J D,Hwa Y,Kim J Y. Essential oil and 1,8-cineole from Artemisia lavandulaefolia induces apoptosis in KB cells via mitochondrial stress and caspase activation[J]. Food Science and Biotechnology,2010,19(1):185-191.
[2]Bao X L,Yuan H H,Wang C Z,et al. Antitumor and immunomodulatory activities of a polysaccharide from Artemisia argyi[J]. Carbohydrate Polymers,2013,98(1):1 236-1 243.
[3]Wang G F,Shi L P,Ren Y D,et al. Anti-hepatitis B virus activity of chlorogenic acid,quinic acid and caffeic acid in vivo and in vitro[J]. Antiviral Research,2009,83(2):186-190.
[4]Li Y L,But P P H,Ooi V E C. Antiviral activity and mode of action of caffeoylquinic acids from Schefflera heptaphylla(L.)Frodin[J]. Antiviral Research,2005,68(1):1-9.
[5]Shi H T,Dong L,Jiang J,et al. Chlorogenic acid reduces liver inflammation and fibrosis through inhibition of toll-like receptor 4 signaling pathway[J]. Toxicology,2013,303:107-114.
[6]Ji L L,Jiang P,Lu B,et al. Chlorogenic acid,a dietary polyphenol,protects acetaminophen-induced liver injury and its mechanism[J]. The Journal of Nutritional Biochemistry,2013,24(11):1 911-1 919.
[7]Yun N,Kang J W,Lee S M. Protective effects of chlorogenic acid against ischemia/reperfusion injury in rat liver molecular evidence of its antioxidant and anti-inflammatory properties[J]. Journal of Nutritional Biochemistry,2012,23(10):1 249-1 255.
[8]Yuan X Y,Gao M Z,Xiao H B,et al. Free radical scavenging activities and bioactive substances of Jerusalem artichoke(Helianthus tuberosus L. )leaves[J]. Food Chemistry,2012,133(1):10-14.
[9]Cinkilic N,Cetintas S K,Zorlu T,et al. Radioprotection by two phenolic compounds:Chlorogenic and quinic acid,on X-ray induced DNA damage in human blood lymphocytes in vitro[J]. Food and Chemical Toxicology,2013,53:359-363.
[10]陈育如,钱媛媛,柯小红,等. 一种从野艾蒿中制备咖啡酰奎尼酸的方法:中国,CN201310424422.7[P].2014-02-12
Chen Yuru,Qian Yuanyuan,Ke Xiaohong,et al. A method to prepare caffeoylquinic aicds from Artemisia lavandulaefolia DC:China,CN201310424422.7[P]. 2014-02-12.(in Chinese).
[11]Wu S H,Gong G L,Wang Y Y,et al. Response surface optimization of enzyme-assisted extraction polysaccharides from Dictyophora indusiata[J]. International Journal of Biological Macromolecules,2013,61:63-68.
[12]Zhang J Y,Zhang Q,Li N,et al. Diagnostic fragmention-based and extension strategy coupled to DFIs intensity analysis for identification of chlorogenic acids isomers in Flos Lonicerae Japonicae by HPLC-ESI-MSn[J]. Talanta,2013,104:1-9.
[13]Karioti A,Bolognesi L,Vinciceri F F,et al. Analysis of the constituents of aqueous preparations of stachys recta by HPLC-DAD and HPLC-ESI-MS[J]. Journal of Pharmaceutical and Biomedical Analysis,2010,53(1):15-23.
[14]Clifford M N,Knight S,Kuhnert N. Discriminating between the six isomers of dicaffeoylquinic acid by LC-MSn[J]. Agricultural and Food Chemistry,2005,53:3 821-3 832.

相似文献/References:

[1]杨凤平,李东晓,王建芬,等.美洲一枝黄花中天然产物二咖啡酰奎尼酸和绿原酸的分离纯化研究[J].南京师范大学学报(工程技术版),2016,16(01):084.
　Yang Fengping,Li Dongxiao,Wang Jianfen,et al.The Isolation and Purification of Dicaffeoylquinic Acidsand Chlorogenic Acid in Solidago Canadensis[J].Journal of Nanjing Normal University(Engineering and Technology),2016,16(01):084.

备注/Memo

备注/Memo:
收稿日期:2014-09-22. 通讯联系人:陈育如,博士,教授,研究方向:天然产物与药物. E-mail:chenyuru@njnu.edu.cn.
更新日期/Last Update: 2015-03-30