[1]张玉千,张 宇,龚赛男,等.虎杖中白藜芦醇和虎杖苷的提取工艺研究[J].南京师范大学学报(工程技术版),2020,20(02):088-92.[doi:10.3969/j.issn.1672-1292.2020.02.013]
　Zhang Yuqian,Zhang Yu,Gong Sainan,et al.Study on Extraction Technology of Resveratrol andGlucoside from Polygonum Cuspidatum[J].Journal of Nanjing Normal University(Engineering and Technology),2020,20(02):088-92.[doi:10.3969/j.issn.1672-1292.2020.02.013]
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虎杖中白藜芦醇和虎杖苷的提取工艺研究
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南京师范大学学报（工程技术版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
20卷
期数:
2020年02期
页码:
088-92
栏目:
生物工程
出版日期:
2020-05-15

文章信息/Info

Title:
Study on Extraction Technology of Resveratrol andGlucoside from Polygonum Cuspidatum
文章编号:
1672-1292(2020)02-0088-05
作者:
张玉千; 张 宇; 龚赛男; 许 瑾; 杨阳春
南京师范大学泰州学院化学与生物工程学院,江苏 泰州 225300
Author(s):
Zhang Yuqian; Zhang Yu; Gong Sainan; Xu Jin; Yang Yangchun
School of Chemistry and Bioengineering,Nanjing Normal University Taizhou College,Taizhou 225300,China
关键词:
虎杖苷; 白藜芦醇; 提取; 正交设计
Keywords:
polydatin; resveratrol; extraction; orthogonal design
分类号:
Q939.97
DOI:
10.3969/j.issn.1672-1292.2020.02.013
文献标志码:
A
摘要:
研究同时提取虎杖中虎杖苷和白藜芦醇的最佳工艺. 采用正交设计法优选提取分离条件,借助HPLC法测定虎杖苷和白藜芦醇的含量. 以乙醇为提取溶剂,超声波辅助提取,最优条件为:80%乙醇,料液比为1:20,提取温度为60 ℃,提取时间为40 min,虎杖苷和白藜芦醇的提取含量分别为2.42%和0.41%. 研究结果表明,所选工艺稳定性好,操作简单.
Abstract:
The optimum technology of simultaneously extracting polygonin and resveratrol from polygonum cuspidatum is studied. Orthogonal design method is used to optimize the extraction and separation conditions,and HPLC method is used to determine their contents. The ethanol as extraction solvent,ultrasonic assisted extraction,the optimum condition is 80% ethanol,and solid-liquid ratio of 1:20,extraction temperature of 60 ℃,extracting time for 40 min,giant knotweed glycosides and the extraction of resveratrol content of 2.42% and 0.41% respectively. A conclusion is conducted that the selected process is good in stability, and simple in operation.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2019-04-15.
基金项目:江苏省高校自然科学基金项目(16KJB180017)、2019江苏省大学生实践创新训练项目、特色植物资源天然产物创新产品研发应用项目(NY-011-2015).
通讯作者:张玉千,硕士,讲师,研究方向:天然产物的分离纯化及微生物转化研究. E-mail:zqwl2000@163.com
更新日期/Last Update: 2020-05-15