[1]刘 超,江慧玲,王鸿飞,等.生物与化学转化黄芩苷为黄芩素的研究[J].南京师范大学学报(工程技术版),2021,21(04):056-60.[doi:10.3969/j.issn.1672-1292.2021.04.009]
　Liu Chao,Jiang Huiling,Wang Hongfei,et al.Study on Biological and Chemical Transformation of Baicalin into Baicalein[J].Journal of Nanjing Normal University(Engineering and Technology),2021,21(04):056-60.[doi:10.3969/j.issn.1672-1292.2021.04.009]
点击复制

生物与化学转化黄芩苷为黄芩素的研究
分享到：

南京师范大学学报（工程技术版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
21卷
期数:
2021年04期
页码:
056-60
栏目:
食品科学与工程
出版日期:
2021-12-15

文章信息/Info

Title:
Study on Biological and Chemical Transformation of Baicalin into Baicalein
文章编号:
1672-1292(2021)04-0056-05
作者:
刘 超1; 江慧玲2; 王鸿飞2; 巴塔拿1; 奥斯曼·科拉3; 罗海波1; 陈育如1
(1.南京师范大学食品与制药工程学院,江苏 南京 210023)(2.南京师范大学江苏省微生物与功能基因组学重点实验室,江苏省微生物资源产业化工程技术研究中心,江苏 南京 210023)(3.阿达纳阿尔帕斯兰科技大学工程学院,土耳其 阿达纳 01180)
Author(s):
Liu Chao1; Jiang Huiling2; Wang Hongfei2; Mohamed Hawali Bata Gouda1; Osman Kola3; Luo Haibo1; Chen Yuru1
(1.School of Food Science and Pharmaceutical Engineering,Nanjing Normal University,Nanjing 210023,China)(2.Jiangsu Key Laboratory for Microbes and Functional Genomics,Jiangsu Engineering and Technology Research Center forIndustrialization of Microbial Resources,Nanjing Normal University,Nanjing 210023,China)(3.Faculty of Engineering,Adana Alparslan Türkes Science and Technology University,Adana 01180,Turky)
关键词:
黄芩; 黄芩素; 黄芩苷; 生物转化; 化学转化
Keywords:
Scutellaria baicalensis; baicalein; baicalin; biotransformation; chemical transformation
分类号:
TS201.3
DOI:
10.3969/j.issn.1672-1292.2021.04.009
文献标志码:
A
摘要:
研究了对黄芩根和黄芩茎叶成份的分析和转化,用生物与化学转化方法将黄芩苷转化为黄芩素. 研究发现,生物转化中黑曲霉效果最佳,黄芩苷转化率为89.5%,黄芩素得率为85.8%. 化学酸水解法得到黄芩苷转化率为98.3%,黄芩素得率为51.6%,比传统的直接酸水解法转化率提高了6.2%,简化了酸水解过程,实验重现性更好. 虽然化学转化的转化率略高于生物转化,但生物转化的得率远高于化学转化,且成本更低,过程中无需强酸,对于环境更为友好,绿色安全.
Abstract:
Scutellaria baicalensis,a valuable herb with plentiful flavonoid,root is mainly used as medicine in traditional Chinese medicine. In this paper,the analysis and transformation of components of Scutellaria baicalensis’ roots and stems and leaves are studied. The biological and chemical methods are selected to convert baicalin into baicalein. The results show that Aspergillus Niger has the best biotransformation effect with 89.5% of baicalin conversion rate,while the yield of baicalein is 85.8%. Meanwhile,the conversion rate of baicalin and baicalein are 98.3% and 51.6% by chemical acid hydrolysis,respectively. In addition,the conversion rate of the acid hydrolysis method and the traditional direct acid hydro-lysis method is increased by 6.2%,and the whole process of acid hydrolysis is simplified,which makes it easier to control and the experiment reproducibility is better. These results indicate that although the conversion rate of chemical transformation is slightly higher than that of biotransformation,the yield of biotransformation is much higher than that of chemical transformation,and the cost is lower; no strong acid is needed in the process,which is friendlier to the environment and green and safe. The results of this investigation also provide technical basis for the industrial production of baicalein.

参考文献/References:

[1] 包倩倩,陈西,张淼,等. 黄芩提取物制备工艺的研究进展[J]. 湖南中医杂志,2021,37(7):174-176.
[2]ZHANG L L,CAO B,BAI C K,et al. Predicting suitable cultivation regions of medicinal plants with Maxent modeling and fuzzy logics:a case study of Scutellaria baicalensis in China[J]. Environmental Earth Sciences,2016,75(5):361.
[3]谷婧,黄玮,张文生. 黄芩野生与栽培资源分布调查研究[J]. 中国中医药信息杂志,2013,20(12):42-45.
[4]LIANG J,GUO Q J,CHANG T Y,et al. Reliable origin identification of Scutellaria baicalensis based on terahertz time-domain spectroscopy and pattern recognition[J]. Optik-International Journal for Light and Electron Optics,2018,174:7-14.
[5]YU H S,HAN Y T,LIU C Y,et al. Preparation of baicalein from baicalin using a baicalin-β-D-glucuronidase from Aspergillus niger b.48 strain[J]. Process Biochemistry,2020,97:168-175.
[6]余伊榄,谢国勇,秦民坚. 黄芩叶茶生产过程中杀青工艺探究[J]. 中国野生植物资源,2019,38(2):16-21,28.
[7]LIU X Y,YE Y Y,LIU Q Q,et al. Pharmacological activity and application of roots,stems and leaves of Scutellaria baicalensis Georgi[J]. Medicinal Plant,2019,10(6):4-5.
[8]朱琳,孙红,陈素娥,等. 反相HPLC法同时测定黄芩标准汤剂中黄芩苷、汉黄芩苷和黄芩素的含量[J]. 中医药导报,2021,27(7):65-68.
[9]方誉民,崔孟颖,柳洁,等. 黄芩属植物黄酮生物合成研究进展[J]. 中国中药杂志,2020,45(20):4819-4826.
[10]LIAO H F,YE J,GAO L L,et al. The main bioactive compounds of Scutellaria baicalensis Georgi. for alleviation of inflammatory cytokines:a comprehensive review[J]. Biomedicine & Pharmacotherapy,2021,133(3):110917.
[11]PARK J R,LEE M C,MOON S C,et al. Scutellaria baicalensis Georgi induces caspase-dependent apoptosis via mitogen activated protein kinase activation and the generation of reactive oxygen species signaling pathways in MCF-7 breast cancer cells[J]. Molecular Medicine Reports,2017,16(2):2302-2308.
[12]单慧,杜胤骁,白鹤群,等. 黄芩素的研发进展及其临床药理学[J]. 中国临床药理学与治疗学,2020,25(6):701-708.
[13]ZHAO T T,TANG H L,XIE L,et al. Scutellaria baicalensis Georgi.(Lamiaceae):a review of its traditional uses,botany,phytochemistry,pharmacology and toxicology[J]. Journal of Pharmacy and Pharmacology,2019,71(9):1353-1369.
[14]LONG H,ZHANG H J,DENG A J,et al. Three new lignan glucosides from the roots of Scutellaria baicalensis[J]. Acta Pharmaceutica Sinica B,2016,6(3):229-233.
[15]WANG B M,HUANG T,FANG Q F,et al. Bone-protective and anti-tumor effect of baicalin in osteotropic breast cancer via induction of apoptosis[J]. Breast Cancer Research and Treatment,2020,184(3):711-721.
[16]WANG L Q,CAI C Y,LIU J J,et al. Selective separation of the homologues of baicalin and baicalein from Scutellaria baicalensis Georgi using a recyclable ionic liquid-based liquid-liquid extraction system[J]. Process Biochemistry,2021,103:1-8.
[17]MA X D,ZHANG X G,GUO S J,et al. Application of enzyme-assisted extraction of baicalin from Scutellaria baicalensis Georgi[J]. Preparative Biochemistry & Biotechnology,2020,51(3):241-251.
[18]OOMEN W W,BEGINES P,MUSTAFA N R,et al. Natural deep eutectic solvent extraction of flavonoids of Scutellaria baicalensis as a replacement for conventional organic solvents[J]. Molecules,2020,25(3):617.
[19]郭艳霞,袁莹,王文科. 黄芩的中性甲醇提取物及酶转化的研究[J]. 山西师范大学学报(自然科学版),2018,32(4):57-65.
[20]马宗敏,苗文丽,段绪红,等. 一株发酵转化黄芩苷菌株的筛选及鉴定[J]. 天然产物研究与开发,2019,31(2):230-235.
[21]许闽,肖功胜,杨云. 黄芩素制备工艺研究[J]. 中国实用医药,2008,3(12):3-4.

备注/Memo

备注/Memo:
收稿日期:2021-06-09.
基金项目:江苏省农业科技自主创新资金项目(CX(20)3176)、江苏省政策引导类计划项目(BX2019014).
通讯作者:陈育如,博士,教授,研究方向:植物天然产物与药物. E-mail:chenyuru@njnu.edu.cn
更新日期/Last Update: 2021-12-15