[1]张 云,黄 鹰.粟酒裂殖酵母Atp25蛋白定位和功能研究[J].南京师范大学学报(工程技术版),2021,21(04):080-86.[doi:10.3969/j.issn.1672-1292.2021.04.012]
　Zhang Yun,Huang Ying.Study on the Localization and Function of Atp25 Proteinin Schizosaccharomyces Pombe[J].Journal of Nanjing Normal University(Engineering and Technology),2021,21(04):080-86.[doi:10.3969/j.issn.1672-1292.2021.04.012]
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粟酒裂殖酵母Atp25蛋白定位和功能研究
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南京师范大学学报（工程技术版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
21卷
期数:
2021年04期
页码:
080-86
栏目:
生物工程
出版日期:
2021-12-15

文章信息/Info

Title:
Study on the Localization and Function of Atp25 Proteinin Schizosaccharomyces Pombe
文章编号:
1672-1292(2021)04-0080-07
作者:
张 云; 黄 鹰
南京师范大学生命科学学院,江苏 南京 210023
Author(s):
Zhang Yun; Huang Ying
School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
关键词:
线粒体; 粟酒裂殖酵母; Atp25; 呼吸链
Keywords:
mitochondria; Schizosaccharomyces pombe; Atp25; respiratory chain
分类号:
Q78
DOI:
10.3969/j.issn.1672-1292.2021.04.012
文献标志码:
A
摘要:
通过荧光显微镜观察、线粒体提取、基因敲除和免疫印迹等技术,研究粟酒裂殖酵母Atp25蛋白定位和Atp25蛋白缺失对线粒体功能的影响. Atp25是核基因组编码蛋白质,生物信息学预测和荧光显微镜观察结果显示Atp25蛋白定位在线粒体中,免疫印迹技术进一步验证了Atp25蛋白的定位. 表型研究结果显示,Δatp25菌株存在生长及呼吸缺陷. Δatp25菌株中线粒体呼吸链复合体亚基蛋白(Cox1、Cox2、Cox3、Cob1、Atp6)水平显著降低. 实时荧光定量PCR结果表明,Δatp25菌株中这些复合体亚基的mRNA水平没有变化.
Abstract:
In this paper,we investigate the localization of Atp25 protein in Schizosaccharomyces pombe and the effect of Atp25 protein deletion on mitochondrial function by fluorescence microscopy,mitochondrial extraction,gene knockout and immunoblotting. Atp25 is a nuclear genome-encoded protein. Bioinformatics predictions and fluorescence microscopy show that Atp25 protein is localized in mitochondria. The localization of Atp25 protein is further verified by immunoblotting. Phenotypic studies reveal growth and respiratory defects in strain Δatp25. The levels of mitochondrial respiratory chain complex subunit proteins(Cox1,Cox2,Cox3,Cob1,Atp6)are significantly reduced in strain Δatp25. Real-time fluorescence quantitative PCR results show no change in the mRNA levels of these complex subunits in strain Δatp25.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2021-04-20.
基金项目:国家自然科学基金项目(31400032).
通讯作者:黄鹰,博士,教授,博士生导师,研究方向:微生物学. E-mail:hy858717@163.com
更新日期/Last Update: 2021-12-15