[1]沈美芳,赵文亚,费志良,等.用酶联免疫法测定水产品中氯霉素的残留量[J].南京师范大学学报(工程技术版),2003,03(02):001-4.
　Shen Meifang,Zhao Wenya,Fei Zhiliang,et al.Enzyme Linked Immunosorbent Assay(ELISA) for Quantitative Analysis of Chloramphenicol Residues in Aquatic Products[J].Journal of Nanjing Normal University(Engineering and Technology),2003,03(02):001-4.
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用酶联免疫法测定水产品中氯霉素的残留量
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南京师范大学学报（工程技术版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
03卷
期数:
2003年02期
页码:
001-4
栏目:
出版日期:
2003-06-30

文章信息/Info

Title:
Enzyme Linked Immunosorbent Assay(ELISA) for Quantitative Analysis of Chloramphenicol Residues in Aquatic Products
作者:
沈美芳1;  赵文亚2;  费志良1;  吴光红1;  耿雪冰1
(1. 江苏省水产质量检测中心, 210017, 南京) ( 2. 南京农业大学食品科技学院, 210095, 南京)
Author(s):
Shen Meifang 1;  Zhao Wenya 2;  Fei Zhiliang 1; Wu Guanghong 1; Geng Xuebing 1
1.Aquatic Products Analysis and Testing Center of Jiangsu Province, 210017, Nanjing, PRC)
关键词:
水产品;  氯霉素;  酶联免疫法
Keywords:
aquatic products;  chloramphenicol;  ELISA
分类号:
R155.5
摘要:
运用酶联免疫法测定水产品中的氯霉素残留 .氯霉素浓度在 0. 0 5～ 4. 0 5 μg/kg范围内呈线性 ,线性方程为Y =0 .3 12 11-0 .3 40 14X ,r=0. 994.向样品中分别添加 0 .45、1 .3 5、4 .0 5 μg/kg 3个浓度水平的氯霉素 ,平均回收率分别为84 .5 %、88 .4%和 79 .9% ,最低检测限为 90μg/kg ,批内变异系数为 6 .0 %～ 8 .5 % ,批间变异系数为 5 .8%～ 14 .3 % .结果表明该方法灵敏度高 ,重复性好 ,适合水产品中氯霉素残留筛选检测
Abstract:
Chloramphenicol residues in aquatic products were analyzed by ELISA. The calibration curve was linear in the range of 0.05 ~ 4.05μg/ kg. The average recoveries were 84.5%、88.4% and 79.9% respectively when samples were spiked with 0.45、1.35 and 4105Lg/ kg. The detection limit was 90μg / kg. The intra- assay coefficients of variation were 6.0%~ 8.5% and inter- assay cofficients of variation were 5.8%~ 14.3%. The method is sensitive and suitable for the determination of trace chloramphenicol in aquatic products.

参考文献/References:

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备注/Memo

备注/Memo:
资助项目: 江苏省"十五" 科技攻关项目( BE2001385) 和江苏省科技厅社会公益项目( BM2001704) 资助.
作者简介: 沈美芳, 女, 1968- , 江苏省水产质量检测中心副研究员, 主要从事水产动物营养需要与检测技术的研究.
更新日期/Last Update: 2013-04-29