[1]钱媛媛,陈育如,杨凤平,等.响应面优化野艾蒿二咖啡酰奎尼酸的高效提取[J].南京师范大学学报(工程技术版),2015,15(01):073-77.
 Qian Yuanyuan,Chen Yuru,Yang Fengping,et al.Optimization Extraction of Dicaffeoylquinic Acids from Artemisialavandulaefolia DC.Using Response Surface Methodology[J].Journal of Nanjing Normal University(Engineering and Technology),2015,15(01):073-77.
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响应面优化野艾蒿二咖啡酰奎尼酸的高效提取
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南京师范大学学报(工程技术版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
15卷
期数:
2015年01期
页码:
073-77
栏目:
化学工程
出版日期:
2015-03-20

文章信息/Info

Title:
Optimization Extraction of Dicaffeoylquinic Acids from Artemisialavandulaefolia DC.Using Response Surface Methodology
作者:
钱媛媛1陈育如1杨凤平1柯小红1闫 莽1唐 超2
(1.南京师范大学生命科学学院,江苏省微生物资源产业化工程技术研究中心,江苏省微生物与基因组学重点实验室,江苏省生物多样性与生物技术重点实验室,江苏 南京 210023)(2.滇虹药业集团股份有限公司,云南 昆明 650106)
Author(s):
Qian Yuanyuan1Chen Yuru1Yang Fengping1Ke Xiaohong1Yan Mang1Tang Chao2
(1.School of Life Science,Jiangsu Engineering and Technology Research Centre for Microbiology Resource,Jiangsu Key Laboratory for Biodiversityand Biotechnology,Jiangsu Key Laboratory for Microbes and Functional Genomic,Nanjing Normal University,Nanjing 2
关键词:
野艾蒿二咖啡酰奎尼酸提取响应面LC/MS
Keywords:
Artemisia lavandulaefolia DC.dicaffeoylquinic acidsextractionresponse surface methodologyLC/MS
分类号:
TS202.3
文献标志码:
A
摘要:
采用响应面优化野艾蒿二咖啡酰奎尼酸的提取工艺. 考察乙醇浓度、温度、时间、料液比4个重要因素对二咖啡酰奎尼酸得率的影响. 在乙醇浓度72%、温度53 ℃、提取时间102 min、料液比1:54(g/mL)条件下,二咖啡酰奎尼酸的提取率为91%,得率达3.30%; LC/MS等方法对野艾蒿中二咖啡酰奎尼酸的定量结果表明,野艾蒿中二咖啡酰奎尼酸由3,5-diCQA、4,5-diCQA和1,5-diCQA组成,其含量分别为14.2、10.5和8.3 mg/g,因而野艾蒿是一种二咖啡酰奎尼酸含量丰富的材料.
Abstract:
Response surface methodology is applied to optimize the extraction conditions of diCQAs from Artemisia lavandulaefolia DC. The combined effects of independent variables(ethanol concentration,extraction temperature,extraction time,solid-liquid ratio)are studied and the optimal extraction conditions are as follows:ethanol concentration 72%,extraction temperature 53 ℃,extraction time 102 min,solid-liquid ratio 1:54 g/mL. Under these conditions,the yield of diCQAs is 3.30% and the extraction yield is 91%. The content of diCQAs(3,5-diCQA,4,5-diCQA,1,5-diCQA)are 14.2 mg/g,10.5 mg/g and 8.3 mg/g respectively,which are identified by LC/MS. A.lavandulaefolia DC.is the materials that rich in dicaffeoylquinic acids.

参考文献/References:

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相似文献/References:

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备注/Memo

备注/Memo:
收稿日期:2014-09-22. 通讯联系人:陈育如,博士,教授,研究方向:天然产物与药物. E-mail:chenyuru@njnu.edu.cn.
更新日期/Last Update: 2015-03-30