[1]邹雨叶,王 雪,王雪梅,等.两种多糖复配物的抗氧化性研究[J].南京师范大学学报(工程技术版),2019,19(02):087.[doi:10.3969/j.issn.1672-1292.2019.02.012]
 Zou Yuye,Wang Xue,Wang Xuemei,et al.Study on the Antioxidant Activity of Two Kinds of Polysaccharides Compound[J].Journal of Nanjing Normal University(Engineering and Technology),2019,19(02):087.[doi:10.3969/j.issn.1672-1292.2019.02.012]
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两种多糖复配物的抗氧化性研究
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南京师范大学学报(工程技术版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
19卷
期数:
2019年02期
页码:
087
栏目:
生物工程
出版日期:
2019-06-30

文章信息/Info

Title:
Study on the Antioxidant Activity of Two Kinds of Polysaccharides Compound
文章编号:
1672-1292(2019)02-0087-06
作者:
邹雨叶王 雪王雪梅朱亚男陶明煊
南京师范大学食品与制药工程学院,江苏 南京 210097
Author(s):
Zou YuyeWang XueWang XuemeiZhu YananTao Mingxuan
School of Food Science and Pharmaceutical Engineering,Nanjing Normal University,Nanjing 210097,China
关键词:
香菇多糖苦瓜多糖抗氧化复配比
Keywords:
lentinanmomordica charantiaantioxidantcompounding ratio
分类号:
TS201.4
DOI:
10.3969/j.issn.1672-1292.2019.02.012
文献标志码:
A
摘要:
研究香菇、苦瓜两种多糖复配物的抗氧化性. 采用水提醇沉法获得香菇多糖、苦瓜粗多糖,用Sevage试剂除去蛋白质得到精多糖. 比较两种多糖及其复配物对羟基自由基(·OH)、超氧阴离子自由基(·O2-)、DPPH自由基的清除能力. 香菇多糖和苦瓜多糖复配比例为1:1、1:2、1:3、2:1、2:3、3:1、3:2. 实验结果表明,两种多糖及其不同比例复配物对3种自由基均具有良好的清除能力,当复配比为2:1时,复配物对羟基(·OH)、超氧阴离子(·O2-)、DPPH 3种自由基的清除率的IC50值分别为3.447、2.006、0.500 mg/mL,其清除效果优于两种多糖单独使用或其他比例复配时,此时其抗氧化能力最强.
Abstract:
The paper studies the antioxidation of the polysaccharides of Lentinus edodes and Momordica charantia. The water extraction and alcohol precipitation method are used to obtain the crude polysaccharides of Lentinus edodes and Momordica charantia,and the protein is removed with Sevage reagent to obtain the refined polysaccharide. The scavenging activities of hydroxyl radical(·OH),superoxide anion radical(·O2-)and DPPH radical are compared between two polysaccharides and their complexes. The compounding ratio of polysaccharides of Lentinus edodes and Momordica charantia is 1:1,1:2,1:3,2:1,2:3,3:1,and 3:2. Both polysaccharides and their different proportions of complexes had good scavenging abilities for all three kinds of free radicals. When the compounding ratio is 2:1,the IC50 values of the clearance rates of hydroxy(·OH),superoxide anion(·O2-)and DPPH three radicals are 3.447 mg/mL,2.006 mg/mL,and 0.500 mg/mL respectively,and their clearance effects are better than those of the two polysaccharides and other ratios. Lentinan and momordica charantia polysaccharides has the strongest antioxidant capacity when the compounding ratio is 2:1.

参考文献/References:

[1] 高文庚. 自由基理论在营养保健研究中的作用[J]. 运城学院学报,2003,21(5):42-43.
GAO W G. The role of free radical theory in nutritional health research[J]. Journal of Yuncheng university,2003,21(5):42-43.(in Chinese)
[2]贺淼,黄鑫,李彪,等. 自由基清除物质的研究进展[J]. 中国饲料,2015(1):41-42.
HE M,HUANG X,LI B,et al. Research progress in free radical scavenging substances[J]. China feed,2015(1):41-42.(in Chinese)
[3]陈瑗,周玫,刘尚喜,等. 营养、衰老与自由基理论[J]. 营养学报,2005,27(3):180-183.
CHEN Y,ZHOU M,LIU S X,et al. Nutrition,aging and free radical theory[J]. Acta nutrimenta sinica,2005,27(3):180-183.(in Chinese)
[4]张松.食用菌学[M]. 广州:华南理工大学出版社,2000:153.
ZHANG S. Edible fungi[M]. Guangzhou:South China University of Technology Press,2000:153.(in Chinese)
[5]吕国英,范雷法,张作法,等. 香菇多糖研究进展[J]. 浙江农业学报,2009,21(2):183-188.
Lü G Y,FAN L F,ZHANG Z F,et al. Development of research on lentinan[J]. Acta agriculturae Zhejiangensis,2009,21(2):183-188.(in Chinese)
[6]HOBBS C. Medicinal values of Lentinus edodes(Berk.)Sing.(A-garicomycetideae).A literature review[J]. Int J Med Mush,2000,2:287-297.
[7]张伟敏,霍建聪,张保顺. 苦瓜素性质及其生理功能研究概况[J]. 粮食与油脂,2005(9):44-46.
ZHANG W M,HUO J C,ZHANG B S. Research survey of properties and biological function of momorcharin[J]. Cereals & oils,2005(9):44-46.(in Chinese)
[8]邹美南,魏云,陈军,等. 苦瓜素降血糖作用试验研究[J]. 中国药业,2006,15(4):5-6.
ZOU M N,WEI Y,CHEN J,et al. Study on hypoglycemic action of momordica charantia extracts[J]. China pharmaceuticals,2006,15(4):5-6.(in Chinese)
[9]石雪萍,姚惠源. 苦瓜皂甙降糖机理研究[J]. 食品科学,2008,29(2):366-368.
SHI X P,YAO H Y. Invivo study on hypoglycemic mechanism of momordica charantia L.Saponins[J]. Food science,2008,29(2):366-368.(in Chinese)
[10]陈红漫,李寒雪,阚国仕,等. 苦瓜多糖的抗氧化活性与降血糖作用相关性研究[J]. 食品工业科技,2012,33(18):349-351.
CHEN H M,LI H X,KAN G S,et al. Correlation study between antioxidant activity and lowering blood glucose of momordica polysaccharide[J]. Science and technology of food industry,2012,33(18):349-351.(in Chinese)
[11]钟耀广,林楠,王淑琴,等. 香菇多糖的抗氧化性能与抑菌作用研究[J]. 食品科技,2007,7(40):141-144.
ZHONG Y G,LIN N,WANG S Q,et al. Study on antioxidantive and antimicrobial activities of Lentinan[J]. Food science and technology,2007,7(40):141-144.(in Chinese)
[12]陈敬鑫,张子沛,罗金凤,等. 苦瓜保健功能的研究进展[J]. 食品科学,2012,33(1):271-273.
CHEN J X,ZHANG Z P,LUO J F,et al. Research advances in healthy functions of bitter gourd[J]. Food science,2012,33(1):271-273.(in Chinese)
[13]倪焱,张雄飞,王仲. 2种常用食用菌中多糖化合物复配的体外抗氧化活性研究[J]. 安徽农业科学,2016,44(27):89-92.
NI Y,ZHANG X F,WANG Z. Antioxidant activity of the compound polysaccharides of two common edible fungus[J]. Journal of Anhui agricultural sciences,2016,44(27):89-92(in Chinese)
[14]冯娟. 复配天然多糖的筛选及其降血脂作用[D]. 西安:第四军医大学,2007.
FENG J. The screening of different ratio polysaccharide gum and the effects on decreasing the serum cholesterol and triglyeride level[D]. Xi’an:The Fourth Military Medical University,2007.(in Chinese)
[15]李艳红. 山楂多糖的提取、分离纯化及结构研究[D]. 太原:山西大学,2016.
LI Y H. Study on the extraction,isolation,purification and structure research of Hawthorn fruit polysaccharide[D]. Taiyuan:Shanxi University,2016.(in Chinese)
[16]樊梓鸾,林秀芳,王振宇. 黑木耳多糖与浆果多酚的协同抗氧化研究[J]. 现代食品科技,2015,31(12):166-171.
FAN X L,LIN X F,WANG Z Y. Synergistic antioxidative effect of berry polyphenols with auricularia auricular polysaccharides[J]. Modern food science and technology,2015,31(12):166-171.(in Chinese)
[17]张贺,王倩雯,曹龙奎. 玉米皮多糖的提取及抗氧化性研究[J]. 粮食与油脂,2015,28(6):47-49.
ZHANG H,WANG Q W,CAO L K. Study on extraction of corn peel polysaccharides and its antioxidation[J]. Cereals & oils,2015,28(6):47-49.(in Chinese)
[18]李湘利,刘静,燕伟,等. 芡实多糖的抗氧化性及抑菌特性[J]. 食品与发酵工业,2014,40(11):104-108.
LI X L,LIU J,YAN W,et al. Antioxidant and antibacterial properties of Euryale ferox polysaccharide[J]. Food and fermentation industries,2014,40(11):104-108.(in Chinese)
[19]赵琳静,宋小平,黎方雅. 多糖及其衍生物抗氧化性质的研究进展[J]. 上海工程技术大学学报,2008,22(1):44-47.
ZHAO L J,SONG X P,LI F Y. Research progress on anti-oxidation of polysaccharide and its derivatives[J]. Journal of Shanghai university of engineering science,2008,22(1):44-47.(in Chinese)
[20]GRASSELLI E,COMPALATI A D,VOCI A,et al. Altered oxidative stress/antioxidant status in blood of alcoholic subjects is associated with alcoholic liver disease[J]. Drug and alcohol dependence,2014,143:112-119.
[21]CHEN L H,ZHAO Y,CIECIA R,et al. Effects of alcohol feeding and thiamin deciency on antioxidant defenses in the liver and spleen of rats[J]. Biochemical archives,1999,15(2):103-116.
[22]王秀平,丁仲元. 自由基对人体的影响及其清除方法的研究[J]. 高校理论研究,2008(34):476-477.
WANG X P,DING Z Y. Research on the influence of free radical on human body and its clearing method[J]. Journal of theoretical research,2008(34):476-477.(in Chinese)

备注/Memo

备注/Memo:
收稿日期:2018-06-07.
基金项目:江苏省大学生创新训练计划(201710319047Z)、江苏省研究生科研创新计划(KYCX18-1219).
通讯联系人:陶明煊,副教授,研究方向:生物活性物质与保健功能因子. E-mail:45017@njnu.edu.cn
更新日期/Last Update: 2019-06-30