[1]陈文娟,陈建福,林 艳,等.超声波辅助提取美人蕉叶多酚工艺及其抗氧化活性研究[J].南京师范大学学报(工程技术版),2023,23(03):075-83.[doi:10.3969/j.issn.1672-1292.2023.03.010]
　Chen Wenjuan,Chen Jianfu,Lin Yan,et al.Ultrasonic-Assisted Extraction of Polyphenols From Canna indica Leaves and Its Antioxidant Activity[J].Journal of Nanjing Normal University(Engineering and Technology),2023,23(03):075-83.[doi:10.3969/j.issn.1672-1292.2023.03.010]
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超声波辅助提取美人蕉叶多酚工艺及其抗氧化活性研究
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南京师范大学学报（工程技术版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
23卷
期数:
2023年03期
页码:
075-83
栏目:
食品科学与工程
出版日期:
2023-09-15

文章信息/Info

Title:
Ultrasonic-Assisted Extraction of Polyphenols From Canna indica Leaves and Its Antioxidant Activity
文章编号:
1672-1292(2023)03-0075-09
作者:
陈文娟1; 陈建福2; 3; 林 艳4; 庄远红4
(1.漳州城市职业学院食品工程系,福建 漳州 363000)
(2.漳州职业技术学院石油化工学院,福建 漳州 363000)
(3.福建省精细化工应用技术协同创新中心,福建 漳州 363000)
(4.闽南师范大学生物科学与技术学院,福建 漳州 363000)
Author(s):
Chen Wenjuan1; Chen Jianfu2; 3; Lin Yan4; Zhuang Yuanhong4
(1.Department of Food Engineering, Zhangzhou City Vocational College, Zhangzhou 363000, China)
(2.College of Chemical Engineering, Zhangzhou Institute of Technology, Zhangzhou 363000, China)
(3.Collaborative Innovation Center of Fine Chemicals in Fujian Province, Zhangzhou 363000, China)
(4.School of Biological Science and Biotechnology, Minnan Normal University, Zhangzhou 363000, China)
关键词:
超声波辅助; 美人蕉叶; 多酚; 响应面法; 抗氧化活性
Keywords:
ultrasound-assisted extraction;  Canna indica Leaves;  polyphenols;  response surface methodology;  antioxidant activity
分类号:
TS202
DOI:
10.3969/j.issn.1672-1292.2023.03.010
文献标志码:
A
摘要:
以美人蕉叶为原料,通过单因素试验并结合响应面法,对超声波辅助乙醇溶液提取美人蕉叶多酚的工艺条件进行研究. 选择乙醇浓度、液料比、超声温度和超声时间作为响应面因素,多酚提取率为响应值,确定了能够用于美人蕉叶多酚提取率进行分析和预测的二次多项式回归模型方程. 结果表明,在乙醇浓度为57%、液料比为43 mL/g、超声温度为72 ℃、超声时间为50 min时,测得的美人蕉叶多酚提取率最高,达到18.89 mg/g,与理论上的美人蕉叶多酚提取率(19.15 mg/g)相对误差仅为1.36%,证实了该模型合理,预测可靠. 所提取的美人蕉叶多酚对DPPH自由基和OH自由基具有一定的清除能力并呈现正相关关系,其IC50分别为49.99 mg/L和 315.55 mg/L,证明了美人蕉叶多酚的抗氧化能力.
Abstract:
Taking Canna indica leaves as raw materials, the process conditions of ultrasonic-assisted ethanol solution extraction of polyphenols from Canna indica leaves are studied by single factor experiment combined with response surface methodology. The ethanol concentration, liquid-to-material ratio, ultrasonic temperature and ultrasonic time are selected as the response surface factors, and the polyphenol extraction yield is the response value, and the quadratic polynomial regression model equation that can be used to analyze and predict the polyphenol extraction yield of Canna indica leaves is determined. The results show that when the concentration of ethanol is 57%, the ratio of liquid to material is 43 mL/g, the ultrasonic temperature is 72 ℃ and the ultrasonic time is 50 minutes, the extraction yield of polyphenols from Canna indica leaves is the highest, reaching 18.89 mg/g. The theoretical relative error of Canna indica leaves polyphenol extraction yield(19.15 mg/g)is only 1.36%, which also confirms that the model is reasonable and the prediction is reliable. The extracted polyphenol from Canna indica leaves has a certain scavenging ability on DPPH free radical and OH free radical, and there is a positive correlation. Its IC50 is 49.99 mg/L and 315.55mg/L, respectively, which proves the antioxidant capacity of Canna indica leaves polyphenols.

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备注/Memo

备注/Memo:
收稿日期:2022-07-27.
基金项目:漳州市自然科学基金项目(ZZ2021J27)、福建省中青年教师教育科研项目(JAT210934).
通讯作者:陈建福,博士,副教授,研究方向:食品应用化学. E-mail:qjf1996@163.com
更新日期/Last Update: 2023-09-15