[1]戴亦军,袁生,秦怀兰.深红酵母产生的类胡萝卜素对蛋黄着色的研究[J].南京师范大学学报(工程技术版),2004,04(04):055-57.
　DAI Yijun,YUAN Sheng,QIN Huailan.Yolk Pigmentation of Carotenoid Produced from Rhodotorula rubra[J].Journal of Nanjing Normal University(Engineering and Technology),2004,04(04):055-57.
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深红酵母产生的类胡萝卜素对蛋黄着色的研究
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南京师范大学学报（工程技术版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
04卷
期数:
2004年04期
页码:
055-57
栏目:
出版日期:
2004-12-30

文章信息/Info

Title:
Yolk Pigmentation of Carotenoid Produced from Rhodotorula rubra
作者:
戴亦军; 袁生; 秦怀兰
南京师范大学生命科学学院, 江苏南京210097
Author(s):
DAI Yijun;  YUAN Sheng;  QIN Huailan
School of Life Science, Nanjing Normal University, Nanjing 210097, China
关键词:
深红酵母;  类胡萝卜素;  色价;  着色剂;  营养增补剂
Keywords:
Rhodotorula rubr a;  carotenoid;  color step;  color pigment;  nutritious additive
分类号:
S816
摘要:
用含经破碎细胞壁的深红酵母菌体的饲料进行鹌鹑喂养试验 ,来研究深红酵母的类胡萝卜素对鹌鹑蛋的着色 .随着鹌鹑不断进食深红酵母 ,实验组的蛋黄色价和类胡萝卜素含量不断升高 ,8d后 ,蛋黄的色价从 6级增加到 15级 ,对照组的蛋黄的色素保持在 6级左右 .用丙酮和石油醚提取蛋黄色素 ,进行 3 60～ 60 0nm波长光谱扫描 ,实验组的波形与 β 胡萝卜素的波形基本相同 .用反相高效液相色谱分析蛋黄中 β 胡萝卜素的含量 ,实验组可达到 0 .0 3 2mg/yolk ,比对照组提高了 2 1倍 .实验结果表明 ,深红酵母是一种很好的天然蛋黄着色剂和营养增补剂 .
Abstract:
The experiment has been made with the quails that fed on the food containing the yeast Rhodotorula rubra with the broken cell walls, to study the pigmentation effect of carotenoid from Rhodotorula r ubra on the yolks of quails. The yolk color step and the carotenoid content of the quail subjects gradually increased, with the color step rising from step 6 to step 15 during 8 days, while the normal group of quails kept the color step 6. The spectrum scanning for the pigment of the yolks has been made with the wave lengths from 360 nm to 600 nm, showing that the quail subjects and the normal group of quails are similar in the wave forms. Using the method of reverse HPLC to analyze the β- carotene content, the result has shown that the β- carotene content of the subjects reached 0. 032 mg/ yolk, increased by 21 times compared with the normal group of quails. The exper imental results have proved that the yeast Rhodotorula rubra is a good natural yolk color pigment and nutritious additive.

参考文献/References:

[ 1] 尹兆正, 童莲芳. 蛋黄色素的研究进展[ J] . 中国家禽, 1994, 16( 4) : 32- 33.
[ 2] 晓理. 欧盟将严格限制家禽饲养色素用量[ J] . 中国家禽, 2003, 25( 7) : 45.
[ 3] Hari R P K. A new strain of Rhodotorula rubra isolated from the ypgurt[ J] . J Basic Microbiol, 1993, 33( 6) : 379 -388.
[ 4] Burton G W. Antioxidant action of carotenoids[ J] . Journal of Nutrition, 1989, 119: 109- 111.
[ 5] 王恬. 饲料添加剂应用原理及技术[M] . 南京: 江苏科技出版社, 1994. 258 -261.

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备注/Memo

备注/Memo:
作者简介: 戴亦军( 1972- ) , 硕士, 博士研究生, 讲师, 主要从事微生物酶工程的教学与研究. E-mail : dyj@ email . njnu. edu. cn
通讯联系人: 袁生( 1956- ) , 博士, 教授, 博士生导师, 主要从事微生物分子生物学和微生物酶工程的教学与研究.E-mail : shengyuan@ email . njnu. edu. cn
更新日期/Last Update: 2013-04-29