[1]陆文娟,喻 晨,王美菊,等.响应面法优化提取干巴菌多糖的工艺研究[J].南京师范大学学报(工程技术版),2015,15(03):084.
 Lu Wenjuan,Yu Chen,Wang Meiju,et al.Optimization Study on the Extraction Technology of Polysaccharidefrom T. ganba jun Zang by Response Surface Method[J].Journal of Nanjing Normal University(Engineering and Technology),2015,15(03):084.
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响应面法优化提取干巴菌多糖的工艺研究
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南京师范大学学报(工程技术版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
15卷
期数:
2015年03期
页码:
084
栏目:
生物工程
出版日期:
2015-09-20

文章信息/Info

Title:
Optimization Study on the Extraction Technology of Polysaccharidefrom T. ganba jun Zang by Response Surface Method
作者:
陆文娟喻 晨王美菊陶眀煊
南京师范大学金陵女子学院,江苏 南京 210097
Author(s):
Lu WenjuanYu ChenWang MeijuTao Mingxuan
Ginling College,Nanjing Normal University,Nanjing 210097,China
关键词:
干巴菌多糖提取超声波响应面法
Keywords:
T. ganba jun Zangpolysaccharides extractionultrasound microwaveresponsesurface methodology
分类号:
TS201.4
文献标志码:
A
摘要:
以干巴菌为原料,采用超声细胞破碎法提取其多糖.在单因素实验的基础上,采用响应面法对提取工艺进行优化,通过Box-Behnken设计,建立并分析了各因素与多糖得率关系的数学模型. 结果显示,最佳工艺条件为:液料比为38∶1,提取时间为3 h,提取温度为88 ℃,超声功率为603 W,重复2次,测定干巴菌多糖的得率为5.96%.
Abstract:
With T. ganba jun Zang as raw materials,polysaccharide is extracted by ultrasonic cell disruption methods. Based on single factor experiments,response surface method is adopted to optimize the extraction process. By Box-Behnken design,the paper builds and analyzes a mathematical model of the factors and the relationship about the yield of polysaccharides. The results show that the optimum conditions are:liquid ratio is 38∶1,the extraction time is 3 h,the extraction temperature is 88 ℃,ultrasonic power is 603 W,repeated two times,polysaccharide yield is measured to be 5.96%.

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备注/Memo

备注/Memo:
收稿日期:2015-05-24. 
基金项目:江苏省普通高校研究生科研创新计划项目(KYLX_0729). 
通讯联系人:陶明煊,副教授,研究方向:生物活性物质与保健功能因子. E-mail:45017@njnu.edu.cn
更新日期/Last Update: 2015-09-20