[1]赵 萍,张盈月,周家宏,等.响应面法优化花生中黄曲霉毒素的提取及其在快检评价中的应用[J].南京师范大学学报(工程技术版),2022,22(03):072-82.[doi:10.3969/j.issn.1672-1292.2022.03.010]
　Zhao Ping,Zhang Yingyue,Zhou Jiahong,et al.Optimization of Extraction of Aflatoxin from Peanuts by Response Surface Methodology and Its Application in Rapid Detection and Evaluation[J].Journal of Nanjing Normal University(Engineering and Technology),2022,22(03):072-82.[doi:10.3969/j.issn.1672-1292.2022.03.010]
点击复制

响应面法优化花生中黄曲霉毒素的提取及其在快检评价中的应用
分享到：

南京师范大学学报（工程技术版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
22卷
期数:
2022年03期
页码:
072-82
栏目:
食品科学与工程
出版日期:
2022-09-15

文章信息/Info

Title:
Optimization of Extraction of Aflatoxin from Peanuts by Response Surface Methodology and Its Application in Rapid Detection and Evaluation
文章编号:
1672-1292(2022)03-0072-11
作者:
赵 萍1; 2; 张盈月1; 周家宏3; 陈 飞2; 3
(1.南京师范大学食品与制药工程学院,江苏 南京 210023)(2.南京师范大学南京市特色生物资源功能成分开发工程研究中心,江苏 南京 210023)(3.南京师范大学生命科学学院,江苏 南京 210023)
Author(s):
Zhao Ping1; 2; Zhang Yingyue1; Zhou Jiahong3; Chen Fei2; 3
(1.School of Food Science and Pharmaceutical Engineering,Nanjing Normal University,Nanjing 210023,China)(2.Nanjing Engineering Research Center for Functional Components Development of Featured Biological Resources,Nanjing Normal University,Nanjing 210023,
关键词:
响应面设计; 黄曲霉毒素; 快检试剂盒; 评价验证
Keywords:
response surface design; aflatoxin; quick detection kit; evaluation of validation
分类号:
TS207.3
DOI:
10.3969/j.issn.1672-1292.2022.03.010
文献标志码:
A
摘要:
采用超声辅助响应面法对花生中黄曲霉毒素(Aflatoxin B1/B2/G1/G2,AFTS)的提取工艺进行优化,得到最佳提取工艺为65%甲醇溶液、超声时间15 min、超声功率206 W、料液比1:4(g:mL),利用该工艺,AFTS的提取量可达18.06 μg/kg. 在此最优工艺条件下提取AFTS,并结合高效液相色谱(HPLC)—光化学柱后衍生法作为参考方法,对市场上主流的8家黄曲霉毒素B1定性试剂盒及2家黄曲霉毒素B1定量试剂盒进行质量评价方法的验证,结果表明定性试剂盒检测结果灵敏度为100%,特异性98%,假阴性率0,假阳性率2%; 定量试剂盒准确度及精密度较高,线性拟合度均>0.99,可满足检测需求.
Abstract:
The extraction process of aflatoxin(AflatoxinB1/B2/G1/G2,AFTS)from peanuts is optimized by ultrasound-assisted response surface method. The optimum extraction process is obtained as follows:65% methanol solution,ultrasonic time 15 min,ultrasonic power 206 W,and the ratio of material to liquid 1:4. Under this optimal condition,the extraction of AFTS is 18.06 μg/kg. The peanut samples are pretreated with the best technology and detected by high performance liquid chromatography(HPLC)with photochemical post-column derivation method,which is used as a reference method to evaluate the quality of the rapid detection kit. There are eight quantitative quits and two qualitative quits populared in the market to be evaluated and tested. The results show that the sensitivity of the qualitative kit is 100%, that the specificity is 98%, that the false negative rate is 0,and that the false positive rate is 2%. The accuracy and precision of the quantitative kit are high,and the linear fitting degree is more than 0.99, which can meet the detection requirements.

参考文献/References:

[1]HE S,HUANG Q T,ZHANG Y,et al. Magnetic beads-based multicolor colorimetric immunoassay for ultrasensitive detection of aflatoxin B1[J]. Chinese Chemical Letters,2020,32(4):1462-1465.
[2]GILL C,VAN DE WIJERT J H,BLOW F,et al. Evaluation of lysis methods for the extraction of bacterial DNA for analysis of the vaginal microbiota[J]. PLOS ONE,2016,11(9):e0163148.
[3]YU L L,ZHANG Y,HU C Y,et al. Highly sensitive electrochemical impedance spectroscopy immunosensor for the detection of AFB1 in olive oil[J]. Food Chemistry,2015,176:22-26.
[4]范蓓,李庆鹏,哈益明. 关注黄曲霉毒素国内外限量标准,完善食品安全保障措施[J]. 食品工业科技,2010(12):26-28.
[5]国家卫生和计划生育委员会,国家食品药品监督管理局. 食品安全国家标准 食品中真菌毒素限量:GB 2761—2017[S]. 北京:中国标准出版社,2017.
[6]WILKOWSKA A,BIZIUK M. Determination of pesticide residues in food matrices using the QuEChERS methodology[J]. Food Chemistry,2011,125(3):803-812.
[7]张洪歌. 果蔬食材中剧毒鼠药和氰化物快速检测方法的优化应用[D]. 北京:中国人民公安大学,2019.
[8]GABALDN J A,CASCALES J M,MORIAS S,et al. Determination of atrazine and carbaryl pesticide residues in vegetable samples using a multianalyte dipstick immunoassay format[J]. Food Additives & Contaminants,2003,20(8):707-715.
[9]HEIDENREICH B,PÖHLMANN C,SPRINZL M,et al. Detection of escherichia coli in meat with an electrochemical biochip[J]. Journal of Food Protection,2010,73(11):2025-2033.
[10]谢刚,叶金,王松雪. 食品安全快速检测方法评价技术研究进展[J]. 食品科学,2016,37(17):270-274.
[11]刘婷,曹进,王钢力,等. 市售食品快速检测产品的应用评价与分析[J]. 食品安全质量检测学报,2017,8(7):2439-2447.
[12]GBASHI S,NJOBEH P B,DE SAEGER S,et al. Development,chemometric-assisted optimization and in-house validation of a modified pressurized hot water extraction methodology for multi-mycotoxins in maize[J]. Food Chemistry,2019,307:125526.
[13]DONG H,XIAN Y P,XIAO K J,et al. Development and comparison of single-step solid phase extraction and QuEChERS clean-up for the analysis of 7 mycotoxins in fruits and vegetables during storage by UHPLC-MS/MS[J]. Food Chemistry,2019,274:471-479.
[14]DU L J,CHU C,WARNER E,et al. Rapid microwave-assisted dispersive micro-solid phase extraction of mycotoxins in food using zirconia nanoparticles[J]. Journal of Chromatography A,2018,1561:1-12.
[15]GBASHI S,NJOBEH P,STEENKAMP P,et al. The effect of temperature and methanol-water mixture on pressurized hot water extraction(PHWE)of anti-HIV analogoues from Bidens pilosa[J]. Chemistry Central Journal,2016,10(1):37.
[16]国家卫生和计划生育委员会,国家食品药品监督管理局. 食品安全国家标准 食品中黄曲霉毒素B族和G族的测定:GB 5009.22—2016[S]. 北京:中国标准出版社,2016.
[17]NAKHJAVAN B,AHMED N S,KHOSRAVIFARD M. Development of an improved method of sample extraction and quantitation of multi-mycotoxin in feed by LC-MS/MS[J]. Toxins,2020,12(7):462.
[18]HUANG Z P,HE J,LI H Y,et al. Synthesis and application of magnetic-surfaced pseudo molecularly imprinted polymers for zearalenone pretreatment in cereal samples[J]. Food Chemistry,2019,308:125696.
[19]潘振朝. 花生及花生油中黄曲霉毒素B1检测方法优化及危害分析[D]. 广州:华南农业大学,2016.
[20]国家质量监督检验检疫总局. 商品化食品检测试剂盒评价方法:SN/T 2775—2011[S]. 北京:中国标准出版社,2011.
[21]于筱雨,方佳兴,向琴,等. 响应面法优化郫县豆瓣中有机酸的提取工艺及HPLC定量分析[J]. 食品科学,2019,40(4):286-291.
[22]YU M,WANG B,QI Z,et al. Response surface method was used to optimize the ultrasonic assisted extraction of flavonoids from Crinum asiaticum[J]. Saudi Journal of Biological Sciences,2019,26(8):2079-2084.
[23]WANG L T,LU W H,LI J L,et al. Optimization of ultrasonic-assisted extraction and purification of zeaxanthin and lutein in corn gluten meal[J]. Molecules,2019,24(16):2994.
[24]陈文娟,陈建福. 超声辅助提取血柚皮多糖工艺优化及其对自由基的清除作用[J]. 南京师大学报(自然科学版),2014,37(1):141-146.
[25]CIRIC A,KRAJNC B,HEATH D,et al. Response surface methodology and artificial neural network approach for the optimization of ultrasound-assisted extraction of polyphenols from garlic[J]. Food and Chemical Toxicology,2020,135:110976.
[26]陆文娟,喻晨,王美菊,等. 响应面法优化提取干巴菌多糖的工艺研究[J]. 南京师范大学学报(工程技术版),2015,15(3):84-92.
[27]WU L,QIU L J,ZHANG H J,et al. Optimization for the production of deoxynivalenol and zearalenone by fusarium graminearum using response surface methodology[J]. Toxins,2017,9(2):57.
[28]姚誉阳,顾妍丽,吴宇伉,等. QuEChERS-高效液相色谱-柱后光化学衍生法测定粮谷类食品中黄曲霉毒素[J]. 食品安全质量检测学报,2021,12(9):3497-3502.
[29]LI Q,LU Z C,TAN X C,et al. Ultrasensitive detection of aflatoxin B1 by SERS aptasensor based on exonuclease-assisted recycling amplification[J]. Biosensors and Bioelectronics,2017,97:59-64.
[30]JIA B Y,LIAO X F,SUN C N,et al. Development of a quantum dot nanobead-based fluorescent strip immunosensor for on-site detection of aflatoxin B1 in lotus seeds[J]. Food Chemistry,2021,356:129614.
[31]张威,张文中,郭平,等. 食用油中黄曲霉毒素B1快速检测试纸的评价[J]. 食品科学,2020,41(12):326-331.
[32]程楠,何景,董凯,等. 试纸法在食品安全快速检测中的研究进展[J]. 食品科学,2015,36(1):256-261.
[33]师邱毅,纪其雄,许莉勇. 食品安全快速检测技术及应用[M]. 北京:化学工业出版社,2010.

备注/Memo

备注/Memo:
收稿日期:2021-10-22.
基金项目:“十三五”国家重点研发计划重点专项项目(2018YFC1603400).
通讯作者:陈飞,博士,副教授,研究方向:食品安全. E-mail:chenfei@njnu.edu.cn
更新日期/Last Update: 2022-09-15