|Table of Contents|

Influence of H2O2 on DNA by Raman Spectroscopy(PDF)

南京师范大学学报（工程技术版）[ISSN:1006-6977/CN:61-1281/TN]

Issue:
2006年03期
Page:
83-85
Research Field:
Publishing date:

Info

Title:
Influence of H2O2 on DNA by Raman Spectroscopy
Author(s):
ZHOU Dianfeng~1; KE Weizhong~2; HENG Hang~2; JI Kang~2
1.Department of Physics,Yancheng Normal College,Yancheng 224002,China;2.Jiangsu Provincial Key Lab for Photoelectric Technology,Analysis and Test Centre,Nanjing Normal University,Nanjing 210097,China
Keywords:
ram an spectra;  DNA;  H2O2
PACS:
Q523-3
DOI:
-
Abstract:
The Ram an spec tra o f DNA in aqueous so lution treated by 1w t. - %, 2w t. -% and 3w t. - % H2O2 for 48h arem easured. TheRam an spec tralm easurem ents indicate that DNA in aqueous so lution show s both A - type and B- type conforma tion w ith B- type conform ation in them ajor ity. A - type con fo rm ation o fDNA in aqueous so lution is dom inated afte r treatm ent o fH2O2 for 48h. H2O2 leads to decrease in the base stack ing. H2O2 doesn‘ t dam ag e the bases near ly, and only damages the backbone o fDNA slightly. The dam age of DNA is m a inly from HO resu lted from chem ica l reaction instead ofH 2 O2 itse lf.

References:

[ 1] 刘颂豪, 孟耀勇. 血卟啉单甲醚对DNA 损伤的拉曼光谱研究[ J]. 光学学报, 2000, 20( 4): 529-532.
LIU Songhao, MENG Y aoyong. Phtodynam ic dam ages o f DNA w ith HEME probed by ram an spectro scopy [ J]. Ac ta Optica S in ica, 2000, 20( 4): 529-532. ( in Chinese)
[ 2] 周殿凤, 柯惟中, 顾柏平, 等. 维生素C 对紫外线诱发DNA 损伤的保护作用[ J]. 光学学报, 2005, 25( 5): 643-646.
ZHOU Dian feng, KEW e izhong, GU Bop ing, et a.l Pro tective effect o f vitam in C on u ltrav iolet radiation- induced DNA dam age [ J] . Acta Optica S in ica, 2005, 25( 5): 643-646. ( in Ch inese)
[ 3] PUPPELS G J, OTTO C, GREVE J, et a .l Ram an m icrospec troscop ic study o f low-pH- induced chang es in DNA structure o f po ly tene chrom osom es[ J]. B io chem istry, 1994, 33( 11) : 3384-3391.
[ 4] 欧荣, 邹国林. 维生素C或H2O2
系统中HO· 对DNA的损伤作用[ J]. 武汉大学学报: 自然科学版, 1997, 43( 2): 239- 241.
OU Rong, ZOU Guo lin. DNA dam age byHO·in the sy stem s conta in ing asco rbate o rH 2O2 [ J]. Journa l o fW uhan Un iv ers ity: Na tura l Sc ience Ed ition, 1997, 43( 2): 239-241. ( in Ch inese)
[ 5] 朱志良, 庄志雄, 黄钰. GSH 在H2O2 致DNA损伤中的作用研究[ J] . 现代预防医学, 2002, 29( 4): 518-520.
ZHU Zhiliang, ZHUANG Zhix iong, HUANG Yu. Study on e ffect of GSH on DNA dam ag e due toH2O2 [ J]. M odern Preventive M ed ic ine, 2002, 29( 4): 518-520. ( in Ch inese)
[ 6] 赵红霞, 许以明, 张志义. 竹红菌乙素及其溴代物对DNA结构光敏损伤的Ram an光谱[ J]. 科学通报, 1998, 43( 9): 960-966.
ZHAO H ongx ia, XU Y im ing, ZHANG Zh iy.i The photosensitive dam age of HB and 5-Br-H B to DNA structure [ J]. Ch inese Sc ience Bu lletin, 1998, 43( 9): 960-966. ( in Chinese)

Memo

Memo:
-
Last Update: 2013-04-29