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Study on Inhibitation of the Tube Formation by RNA Introduced by Adenovirus by its Interference With and Decrease of VEGF Transcription(PDF)

南京师范大学学报（工程技术版）[ISSN:1006-6977/CN:61-1281/TN]

Issue:
2007年04期
Page:
84-87
Research Field:
Publishing date:

Info

Title:
Study on Inhibitation of the Tube Formation by RNA Introduced by Adenovirus by its Interference With and Decrease of VEGF Transcription
Author(s):
Xu Jianfeng; Shen Weigan; Peng Wanxin; Li Chaojun
School of Life Science,Nanjing Normal University,Nanjing 210097,China
Keywords:
ang iogenesis;  VEGF;  RNA i
PACS:
Q789
DOI:
-
Abstract:
This study uses the hom o logous recomb ination technique to construct a recomb inan t adenov irusAdH 1-siRNA /VEGF ta rgeting VEGF infects hum an endo the lia l ce lls ( HUVEC ), obse rves the inter fe rence o f the adenov irus w ith the pro liferation ofHUVEC in v itro and the tube fo rm ation. The exper iem ent of rea-l tim e reve rse transcr iption- PCR show s tha t this adenov irus can espec ia lly decrease level o f the VEGF mRNA o fHUVEC. Com pared w ith control g roup, themRNA expression o fVEGF decreased to 50%. AdH 1-siRNA /VEGF can effic iently inte rfere the pro lifeya-t ion o fHUVEC. N ine day s after infection o f adenov irus, the ave rage cell number o f contro l g roup and vector g roup are 31. 5 × 104 /LL and 28 × 104 /LL respective ly, that o f groups respective ly infected w ith AdH 1-siRNA /VEGF are 21. 5 × 104 /LL, and can effic iently inte rfere the tube form ation o fHUVEC onM aty ig e.l Ave rage ly, the tube number of everyH PF is 10. 75 in the contro l g roup, and 10. 25 in the vecto r group. In the group treated w ith AdH 1-siRNA / VEGF, there are 7 tubes in theHPE. AdH 1-siRNA /VEGF can inte rfere tube form a tion.

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Last Update: 2013-04-29