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Studies on the Function of Mmp1 Protein in Schizosaccharomyces Pombe(PDF)

南京师范大学学报（工程技术版）[ISSN:1006-6977/CN:61-1281/TN]

Issue:
2022年04期
Page:
69-74
Research Field:
生物工程
Publishing date:

Info

Title:
Studies on the Function of Mmp1 Protein in Schizosaccharomyces Pombe
Author(s):
Zhao Lan; Huang Ying
(School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)
Keywords:
Mmp1; mitochondria; qRT-PCR; mitochondrial protein extraction
PACS:
Q78
DOI:
10.3969/j.issn.1672-1292.2022.04.009
Abstract:
Mitochondria are the productive organelles of most eukaryotic cells,which are involved in various physiological processes such as ATP synthesis,substance metabolism and cell apoptosis. Mmp1 protein is predicted to be the catalytic subunit of the mitochondrial endopeptidase complex,but its specific function has not been analyzed. In this paper,the localization of Mmp1 and its effect on mitochondrial function are analyzed by molecular cloning,phenotypic experiment,fluorescence microscopic observation,mitochondrial isolation and extraction,real-time fluorescence quantitative PCR and western-blot. Fluorescence localization analysis reveal that Mmp1 is a protein located in mitochondria. Δmmp1 strain can not grow on the medium with glycerol as carbon source and can not perform aerobic respiration,suggesting that the deletion of mmp1 affects mitochondrial function. Meanwhile,the protein levels of Cox1,Cox2,Cox3,Cob1 and Atp6,the core proteins of respiratory chain complex encoded by mitochondrial genome,are significantly reduced when mmp1 is knock out. The transcriptional levels of Cox1,Cox2,Cox3,Cob1 and Atp6 in Δmmp1 strain do not change significantly. These results indicate that Mmp1 affects the protein levels of some mitochondrial respiratory chain complex subunits and plays an important role in maintaining mitochondrial function.

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Last Update: 2022-12-15