[1]杨凤平,李东晓,王建芬,等.美洲一枝黄花中天然产物二咖啡酰奎尼酸和绿原酸的分离纯化研究[J].南京师范大学学报(工程技术版),2016,16(01):084.
 Yang Fengping,Li Dongxiao,Wang Jianfen,et al.The Isolation and Purification of Dicaffeoylquinic Acidsand Chlorogenic Acid in Solidago Canadensis[J].Journal of Nanjing Normal University(Engineering and Technology),2016,16(01):084.
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美洲一枝黄花中天然产物二咖啡酰奎尼酸和绿原酸的分离纯化研究
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南京师范大学学报(工程技术版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
16卷
期数:
2016年01期
页码:
084
栏目:
生物工程
出版日期:
2016-03-31

文章信息/Info

Title:
The Isolation and Purification of Dicaffeoylquinic Acidsand Chlorogenic Acid in Solidago Canadensis
作者:
杨凤平李东晓王建芬陈育如
南京师范大学生命科学学院,江苏省微生物资源产业化工程技术研究中心,江苏省微生物与基因组学重点实验室,江苏省生物多样性与生物技术重点实验室,江苏 南京 210023
Author(s):
Yang FengpingLi DongxiaoWang JianfenChen Yuru
School of Life Science,Jiangsu Engineering and Technology Research Centre for Microbiology Resource,Jiangsu Key Laboratory for Microbes andFunctional Genomic,Jiangsu Key Laboratory for Biodiversity and Biotechnology,Nanjing Normal University,Nanjing 210023,China
关键词:
美洲一枝黄花二咖啡酰奎尼酸绿原酸分离纯化大孔树脂
Keywords:
Solidago Canadensisdicaffeoylquinic acidschlorogenic acidisolation and purificationmacroporous resin
分类号:
TS202.3
文献标志码:
A
摘要:
研究了入侵植物美洲一枝黄花中的成分,分析鉴定了其中重要成分二咖啡酰奎尼酸和绿原酸的含量并探讨了其分离纯化. 美洲一枝黄花不同部位中以花和叶的二咖啡酰奎尼酸和绿原酸有效成分含量最高,其花蕾中的二咖啡酰奎尼酸和绿原酸的含量最高分别达1 530 mg/100 g和700 mg/100g. 以60%乙醇为提取剂,在固液比1∶30,50?℃超声作用下提取1 h可高效提取有效成分,绿原酸和二咖啡酰奎尼酸的提取率分别达90.3%和91.6%. 提取液经大孔树脂CN300用40%~50%和50%~70%的乙醇溶液洗脱,可有效分离提取液中的二咖啡酰奎尼酸与绿原酸,再经乙酸乙酯萃取纯化后可分别得到绿原酸和二咖啡酰奎尼酸产品.
Abstract:
In this work,the natural compounds of the invasive plant Solidago Canadensis are studied,and the contents of dicaffeoylquinic acids and chlorogenic acid are analyzed. The separation and purification process are discussed. The contents of dicaffeoylquinic acids and chlorogenic acid are the highest in flower and leaf of Solidago Canadensis. The contents of dicaffeoylquinic acids and chlorogenic acid reach 1 530 mg/100 g and 700 mg/100 g in the bud of Solidago Canadensis respectively. The extraction rates of chlorogenic acid and dicaffeoylquinic acids respectively reach 90.3% and 91.6% under the optimal extraction conditions:ethanol concentration 60%,extraction temperature 50 ℃,extraction time 1 h,and solid-liquid ratio 1∶30 g/mL. The dicaffeoylquinic acids and chlorogenic acid are effectively extracted by the macroporous resin CN300 with 40%~50% and 50%~70% ethanol solution,and then having been extracted by ethyl acetate,chlorogenic acid and dicaffeoylquinic acids are obtained respectively.

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相似文献/References:

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 Qian Yuanyuan,Chen Yuru,Yang Fengping,et al.Optimization Extraction of Dicaffeoylquinic Acids from Artemisialavandulaefolia DC.Using Response Surface Methodology[J].Journal of Nanjing Normal University(Engineering and Technology),2015,15(01):073.

备注/Memo

备注/Memo:
收稿日期:2015-12-20. 
基金项目:江苏省“六大人才高峰”人才培养项目(NY-011-2015)、江苏省高校自然科学研究重大项目(15KJA210002). 
通讯联系人:陈育如,博士,教授,研究方向:天然产物与药物研究. E-mail:chenyuru@njnu.edu.cn.
doi:10.3969/j.issn.1672-1292.2016.01.014
更新日期/Last Update: 2016-03-20