[1]叶爱英,张培培,姚 成.植物油沥青中总甾醇的分析[J].南京师范大学学报(工程技术版),2007,07(02):045-48.
　Ye Aiying,Zhang Peipei,Yao Cheng.Determination of Total Sterol in Plant Oil Pitch[J].Journal of Nanjing Normal University(Engineering and Technology),2007,07(02):045-48.
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植物油沥青中总甾醇的分析
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南京师范大学学报（工程技术版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
07卷
期数:
2007年02期
页码:
045-48
栏目:
出版日期:
2007-06-30

文章信息/Info

Title:
Determination of Total Sterol in Plant Oil Pitch
作者:
叶爱英1;  张培培2;  姚 成2
1. 常州工程职业技术学院应用化学技术系, 江苏常州213164; 2. 南京工业大学理学院, 江苏南京210009
Author(s):
Ye Aiying1; Zhang Peipei 2; Yao Cheng2
1.Department of Applied Chemistry,Changzhou Institute of Engineering Technology,Changzhou 213164,China;2.College of Sciences,Nanjing University of Technology,Nanjing 210009,China
关键词:
植物油沥青;  分光光度法;  甾醇;  甾醇酯
Keywords:
p lant o il pitch;  spectrophotom e try;  stero ; l phy to ste ro l este rs
分类号:
TQ645.98
摘要:
采用改进的Lieberman-Burchard比色法,建立了测定植物油沥青中总甾醇含量的方法.采用不加显色剂的样品溶液作空白,甾醇含量在0.034~0.344 mg/mL范围内服从比耳定律,线性良好.测得植物油沥青中总甾醇含量在12.1%左右,提取物中总甾醇含量为74.5%左右.标准偏差s=0.060 3%,样品回收率在94.8%~98.1%之间.该法操作简单,检验费用低,显色反应明显,并且能稳定0.5 h,适于企业化验室操作,能对植物油沥青中高附加值组分的开发应用提供参考.
Abstract:
The determ ination o f to ta l stero l content in p lant o il pitch w as studied by improved Lieberm an-Burchard co ler ime try. The sam ple so lution w ithout chrom ogenic agent w as used as the blank so lution, and the stero l content obeyed L ieberm an-Burchard law in the range o f 0. 034~ 0. 344 mg /mL w ith good linear ity. The to ta l content o f stero l in plant oil pitch w as abou t 12.1% and in the ex traction the content of stero l w as abou t 74.5%. The SD was 0.0603%; the sam ple recovery ra tew as betw een 94.8% and 98. 1%. Th ism ethod w as s imp le and the detec tion was co sts low. The color reaction was distinc t, and co lo r stab iliza tion tim e was 015 hour. Them ethod wou ld be suitable for corporations. laboratory, and offers it im po rtant re ference for the application o f high-va lue com ponents in the p itch.

参考文献/References:

[ 1] 曹栋, 裘爱永. 植物油中甾醇的快速分析[ J]. 食品与发酵工业, 1989( 6) : 60- 62.
Cao Dong, Q iu A iyong. Quick analysis o f ste ro l in vege tab le o il [ J]. Food and Ferm entation Industr ies, 1989( 6): 60- 62. ( in Chinese)
[ 2] W ester I. Fa t com pos ition for use in food: USA, 6162483[ P] . 2000- 12- 19.
[ 3] 郭玉宝, 裘爱永. 植物甾醇酯降血脂作用的研究[ J]. 中国油脂, 2003, 28( 9): 49- 51.
GuoYubao, Q iu A iyong. Study on b lood-fat- lowe ring effect of phy to ste ry l este rs [ J]. Ch ina O ils and Fats, 2003, 28( 9): 49- 51. ( in Ch inese)
[ 4] 姚成, 叶爱英. 一种从植物油沥青中提取植物甾醇乙酸酯的方法: 中国, CN1763068[ P] . 2006.
Yao Cheng, YeA iy ing. Ex tract o f phy toste ro l ace tates in p lant o il pitch: Ch ina, CN1763068[ P]. 2006. ( in Chinese) [ 5] 姚成, 叶爱英, 徐永林, 等. 一种从植物油沥青中提取植物甾醇的方法: 中国, CN1807444A[ P]. 2006.
Yao Cheng, YeA iy ing, Xu Yong lin, e t a.l Ex tract of phy toste ro l in p lant o il pitch: Ch ina, CN1807444A [ P]. 2006. ( in Ch-i nese)
[ 6] 高瑜莹, 裘爱永, 潘秋琴, 等. 植物甾醇的分析方法[ J]. 中国油脂, 2001, 26( 1) : 25- 28.
Gao Yuy ing, Q iu A iyong, Pan Q iuq in, e t a.l The ana ly sis of phytostero ls[ J]. Ch ina O ils and Fa ts, 2001, 26( 1): 25- 28. ( in Chinese)
[ 7] Kn ights B A. Identification of plant stero ls using com bined GLC /M ass spectrom e try [ J] . J Gas Chrom atogr, 1967( 5): 273.
[ 8] 王三永. 含脂肪食品中植物甾醇的气相色谱法测定[ J] . 分析测试学报, 2001, 20( 4): 42- 43.
W ang Sanyong. Gas chrom atograph ic dete rm ina tion o f plant ste ro ls in fa tty food [ J]. Journa l of Instrum en tal Ana ly sis, 2001, 20( 4): 42- 43. ( in Ch inese)
[ 9] 周宝兰. 油脂中无甲基甾醇定量分析方法的研究: 薄层色谱) 氯胺T 浓硫酸比色法[ J] . 中国粮油学报, 1993, 8 ( 4): 31- 35.
Zhou Bao lan. S tudy on the m ethod for quantitative analysis o f dem eth lyate stero l in refuse of refined o i:l a thin lay er chrom atography ( TLC )-co lor im etr ic m ethod[ J]. Journa l of the Chinese Ce rea ls and O ils A ssoc iation, 1993, 8( 4 ): 31 - 35. ( in Ch-i nese)
[ 10] Carer iM, E lv ir i L, M ang ia A. L iquid chrom a tog raphy) UV determ ination and liqu id chrom atography-a tm ospher ic pressure chem ical ion ization m ass spectrome tr ic character ization o f s itostero l and stigm aste ro l in soybean o il [ J] . Journal of Chrom atography A, 2001, 935: 249- 257.
[ 11] P lank C. Ana ly sis of free and esterified stero ls in vege tab le o ilm ethy l esters by capilla ry GC [ J]. HRC, 1993, 16( 8) : 483 - 490.
[ 12] Ab id i S. L- chrom atogragh ic ana lysis o f plant ste ro l in foods and vegetable o ils [ J]. J Chrom atog rA, 2001, 935( 2): 173- 201.

备注/Memo

备注/Memo:
基金项目: 江苏省农业科技攻关重大课题( BE2006322 )资助项目.
作者简介: 叶爱英( 1971-) , 女, 助教, 主要从事天然产物分析和分离的教学与研究. E-m ail:ayye@ em a il.czie. net
通讯联系人: 姚 成( 1961-) , 教授, 博士生导师, 主要从事分析和合成的教学与研究. E-m ail:yaocheng@ n ju t. edu. cn
更新日期/Last Update: 2013-04-29