[1]戴郁菁,顾晓天.DL-N-乙酰高半胱氨酸内酯的液相色谱分析[J].南京师范大学学报(工程技术版),2008,08(03):047-49.
　Dai Yujing,Gu Xiaotian.Analysis of DL-N-Acetylhomocysteine Thiolactone by HPLC[J].Journal of Nanjing Normal University(Engineering and Technology),2008,08(03):047-49.
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DL-N-乙酰高半胱氨酸内酯的液相色谱分析
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南京师范大学学报（工程技术版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
08卷
期数:
2008年03期
页码:
047-49
栏目:
出版日期:
2008-09-30

文章信息/Info

Title:
Analysis of DL-N-Acetylhomocysteine Thiolactone by HPLC
作者:
戴郁菁;顾晓天;
南京师范大学分析测试中心, 江苏南京210097
Author(s):
Dai Yujing; Gu Xiaotian
Analysis and Testing Center,Nanjing Normal University,Nanjing 210097,China
关键词:
DL-N-乙酰高半胱氨酸内酯;  高效液相色谱法;  药品检验
Keywords:
DL-N-acety lhom ocysteine th io lactone;  HPLC;  m edic ine ana lysis
分类号:
R927
摘要:
建立乙酰高半胱氨酸内酯含量测定的HPLC方法.采用C18柱,流动相为甲醇-水(10∶90),在237 nm下检测,流速为1.0 mL/m in,平均回收率为99.5%.说明使用该方法测定DL-N-乙酰高半胱氨酸内酯,简便快捷,结果准确,适用于原料及制剂的质量控制.
Abstract:
An H PLC m ethod w as deve loped to de term ine the content of DL-N-acety lhom ocy ste ine thio lactone. A C18 co lum n w as used. Them obile phase consisted o f 10% m e thano l and 90% wa ter. The detec tion was 237 nm and the flow ra tew as 1.0 mL /m in. The average recovery o f DL-N-ace ty lhomocyste ine th io lactone w as 99.5%. Therefore, th is m ethod w as easy, qu ick and accurate, and can be used to contro l the qua lity o f DL-N-acety lhom ocy ste ine th io lac tone in raw m ater ia ls and the related products

参考文献/References:

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H an X iao jie, H uang Zhesu, L iH a isheng, et a .l De term ina tion o f acety lcyste ine and its related substances in injection by reversed phase ion pa ir chrom atography[ J]. T ian jin Pharm acy, 2005, 17( 6) : 7. ( in Ch inese)
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Br itish Pharmacopoe ia Comm iss ion. B ritish Pharm acopoe ia[M ] . London: S tationery O ffice, 2003: 2069. ( in Ch inese)
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Un ited States Pharm acopoe ia Comm ission. US Pharm ocopoeia [M ]. Ch icago: The Pha rmaceutica l Press, 2000: 24. ( in Chinese)

备注/Memo

备注/Memo:
通讯联系人: 戴郁菁, 讲师, 研究方向: 仪器分析和表面化学. E-m ail:d aiyu jing@ n jnu. edu. cn
更新日期/Last Update: 2013-04-24