[1]柯小红,陈育如,赵文文,等.多裂翅果菊提取物中菊苣酸等成分的分离纯化[J].南京师范大学学报(工程技术版),2015,15(02):088.
 Ke Xiaohong,Chen Yuru,Zhao Wenwen,et al.Separation and Purification of Cichoric Acid in Pterocypsela laciniata(Houtt. )Shih Extracts[J].Journal of Nanjing Normal University(Engineering and Technology),2015,15(02):088.
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多裂翅果菊提取物中菊苣酸等成分的分离纯化
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南京师范大学学报(工程技术版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
15卷
期数:
2015年02期
页码:
088
栏目:
生物工程
出版日期:
2015-06-20

文章信息/Info

Title:
Separation and Purification of Cichoric Acid in Pterocypsela laciniata(Houtt. )Shih Extracts
作者:
柯小红1陈育如1赵文文1钱媛媛1戴科伟2唐 超3
(1.南京师范大学生命科学学院,江苏省微生物资源产业化工程技术研究中心,江苏省微生物与基因组学重点实验室,江苏省生物多样性与生物技术重点实验室,江苏 南京 210023) (2.南京师范大学地理科学学院,江苏 南京 210023) (3.滇虹药业集团股份有限公司,云南 昆明 650106)
Author(s):
Ke Xiaohong1Chen Yuru1Zhao Wenwen1Qian Yuanyuan1Dai Kewei2Tang Chao3
(1.School of Life Science,Jiangsu Engineering and Technology Research Centre for Microbiology Resource,Jiangsu Key Laboratory for Biodiversity and Biotechnology,Jiangsu Key Laboratory for Microbes and Functional Genomic,Nanjing Normal University,Nanjing 210023,China) (2.School of Geography Science,Nanjing Normal University,Nanjing 210023,China) (3.Dianhong Pharmaceutical Group Co.,LTD,Kunming 650106,China)
关键词:
多裂翅果菊菊苣酸大孔树脂聚酰胺灯盏乙素
Keywords:
Pterocypsela laciniatacichoric acidmacroporous resinpolyamidescutellarin
分类号:
R284.2; R286.02
文献标志码:
A
摘要:
采用CN101大孔树脂对多裂翅果菊提取物中的菊苣酸、灯盏乙素、单咖啡酰酒石酸、绿原酸和3,5-二咖啡酰奎尼酸等5种成分进行分离,分别使用15%和60%的乙醇作为洗脱剂,可成功地将菊苣酸与其他成分分离. 经聚酰胺二次层析,可将菊苣酸的纯度提高至72.0%. 本工作将多裂翅果菊提取物中的菊苣酸浓度(2.53%)提高28.5倍,回收率达到91.4%,同时还可分离单咖啡酰酒石酸、绿原酸、灯盏乙素作为副产品.
Abstract:
A method is developed to separate five active ingredients:cichoric acid,scutellarin,caftartic acid,chlorogenic acid and 3,5-dicaffeoylquinic acid from the extracts of Pterocypsela laciniata(Houtt. )Shih with CN101 macroporous resin,by eluting with 15% ethanol and then 15% ethanol. The purity of cichoric acid is increased to 72% with a second separation by polyamide. Cichoric acid content is 28.5 times more than that 2.53% of the extracts of Pterocypsela laciniata and the recovery rate is 91.4%. Caftartic acid,chlorogenic acid and scutellarin can be obtained as by-products.

参考文献/References:

[1] Bai Yixiao,Tan Jing,Yan Fulin,et al. A new guaianolide from the roots of Pterocypsela elata[J]. Chinese Chemical Letters,2013,24:55-56.
[2]Han Yifeng,Cao Guixiu,Gao Xiaojing,et al. Isolation and characterisation of the sesquiterpene lactones from Lactuca sativa L var.Anagustata[J]. Food Chemistry,2010,120(4):1 083-1 088.
[3]Hui W H,Lee W K. Triterpenoid and steroid constituents of some lactuca and ageratum species of Hong Kong[J]. Phytochemistry,1971,10(4):899-901.
[4]董丽,孙祥德,郭兰青,等. 多裂翅果菊的挥发油成分[J]. 广西植物,2004,24(1):61-63.
Dong Li,Sun Xiangde,Guo Lanqing,et al. The constituents of volatile oil from Pterocypsela laciniata[J]. Guihaia,2004,24(1):61-63.(in Chinese)
[5]侯彩婷,王瑞华,邹昀员,等. UPLC-MS/MS法测定多裂翅果菊中植物甾醇的含量[J]. 食品科学,2012,34(12):301-305.
Hou Caiting,Wang Ruihua,Zou Yunyuan,et al. Analysis of phytosterol content in Pterocypsela laciniata(Houtt. )shih by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Food Science,2012,34(12):301-305.(in Chinese)
[6]陈育如,柯小红,闫莽,等. 以翅果菊为原料同时提取菊苣酸,单咖啡酰酒石酸,3,5-二咖啡酰奎尼酸和绿原酸的方法:中国,CN201310442563.1[P]. 2013.
Chen Yuru,Ke Xiaohong,Yan Mang,et al. A method about the extract of cichoric acid,caftartic acid,3,5-dicaffeoylquinic acid and chlorogenic acid from Pterocypsela:China,CN201310442563.1[P]. 2013.(in Chinese)
[7]Tsai Yul-Ling,Chiu Chien-Chih,Chen Jeff Yi-Fu,et al. Cytotoxic effects of Echinacea purpurea flower extracts and cichoric acid on human colon cancer cells through induction of apoptosis[J]. Journal of Ethnopharmacology,2012(143):914-919.
[8]Line Thygesen,Johanna Thulin,Alan Mortensen. Antioxidant activity of cichoric acid and alkamides from Echinacea purpurea,alone and combination[J]. Food Chemistry,2007(101):74.
[9]Fusco Dahlene,Liu Xinyan,Savage Caroline,et al. Echinacea purpurea aerial extract alters course of influenza infection in mice[J]. Vaccine,2010(28):3 956-3 962.
[10]徐建忠,史秋梅,倪耀娣,等. 紫锥菊药理作用的研究现状[J]. 黑龙江畜牧兽医:科技版,2010(4):41-42.
Xu Jianzhong,Shi Qiumei,Ni Yaodi,et al. The current research of pharmacological effects of Echinacea purperea[J]. Heilongjiang Animal Husbandry and Veterinary:Science and Technology Edition,2010(4):41-42.(in Chinese)
[11]Wang Xiao,Geng Yanling,Li Fuwei,et al. Preparative separation of cichoric acid from Echinacea Purpurea by pH-zone-refining counter-current chromatography[J]. Journal of Chromatography A,2006,1 103(1):166-169.
[12]谢春燕,徐新军,谢鸷生,等. 快速制备液相色谱分离紫锥菊中咖啡酰基酒石酸、菊苣酸和松果菊苷[J]. 中国新药与临床药理,2012,23(1):90-94.
Xie Chunyan,Xu Xinjun,Xie Zhisheng,et al. Isolation of caftaric acid,cichoric acid and echinacoside from Echinacea purpurea by flash chromatography[J]. Traditional Chinese Drug Research & Clinical Pharmacology,2012,23(1):90-94.(in Chinese)
[13]刘轶琛,曾建国,陈波,等. 聚酰胺对紫锥菊提取物中菊苣酸的分离纯化研究[J]. 中草药,2007,38(4):510-532.
Liu Yichen,Zeng Jianguo,Chen Bo,et al. Isolation and purification of cichoric acid from extracts of Echinacea purpurea by polyamide column chromatography[J]. Chinese Traditional and Herbal Drugs,2007,38(4):510-532.(in Chinese)
[14]曾栋,陈波,罗旭彪,等. 大孔吸附树脂对紫锥菊提取物中菊苣酸分离纯化的研究[J]. 天然产物研究与开发,2004,16(2):160-162.
Zeng Dong,Chen Bo,Luo Xubiao,et al. Study on adsorption and purification of cichoric acid in extracts of Echinacea purpurea with macroporous adsorption resin[J]. Natural Product Reasearch and Development,2004,16(2):160-162.(in Chinese)

备注/Memo

备注/Memo:
收稿日期:2014-11-28.
通讯联系人: 陈育如,博士,教授,研究方向:天然产物与药物. E-mail:chenyuru@njnu. edu. Cn
更新日期/Last Update: 2015-06-20