[1]周殿凤,柯惟中,衡航,等.H2O2对DNA影响的拉曼光谱分析[J].南京师范大学学报(工程技术版),2006,06(03):083-85.
　ZHOU Dianfeng~,KE Weizhong~,HENG Hang~,et al.Influence of H2O2 on DNA by Raman Spectroscopy[J].Journal of Nanjing Normal University(Engineering and Technology),2006,06(03):083-85.
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H2O2对DNA影响的拉曼光谱分析
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南京师范大学学报（工程技术版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
06卷
期数:
2006年03期
页码:
083-85
栏目:
出版日期:
2006-09-30

文章信息/Info

Title:
Influence of H2O2 on DNA by Raman Spectroscopy
作者:
周殿凤;柯惟中;衡航;籍康;
周殿凤1, 柯惟中2, 衡 航2, 籍 康2
Author(s):
ZHOU Dianfeng~1; KE Weizhong~2; HENG Hang~2; JI Kang~2
1.Department of Physics,Yancheng Normal College,Yancheng 224002,China;2.Jiangsu Provincial Key Lab for Photoelectric Technology,Analysis and Test Centre,Nanjing Normal University,Nanjing 210097,China
关键词:
拉曼光谱;  DNA;  H2O2
Keywords:
ram an spectra;  DNA;  H2O2
分类号:
Q523-3
摘要:
检测了鲱精DNA水溶液及其经过1%、2%和3%H2O2处理48h的拉曼光谱图.实验结果表明DNA在水溶液中同时具有A、B两种构象,但以B型构象为主.经H2O2处理后,DNA水溶液的A型构象有所增强,碱基堆积程度下降.H2O2对碱基和脱氧核糖几乎没有损伤,对DNA的主链结构也只造成很轻微的损伤.由此可见H2O2本身对DNA的损伤是很小的,只有在外界因素诱发下H2O2经反应生成HO.才会给DNA造成严重损伤.
Abstract:
The Ram an spec tra o f DNA in aqueous so lution treated by 1w t. - %, 2w t. -% and 3w t. - % H2O2 for 48h arem easured. TheRam an spec tralm easurem ents indicate that DNA in aqueous so lution show s both A - type and B- type conforma tion w ith B- type conform ation in them ajor ity. A - type con fo rm ation o fDNA in aqueous so lution is dom inated afte r treatm ent o fH2O2 for 48h. H2O2 leads to decrease in the base stack ing. H2O2 doesn‘ t dam ag e the bases near ly, and only damages the backbone o fDNA slightly. The dam age of DNA is m a inly from HO resu lted from chem ica l reaction instead ofH 2 O2 itse lf.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介: 周殿凤( 1978-) , 女, 助教, 主要从事拉曼光谱研究. E-m ail:zhoud ianfeng@ sohu. com
更新日期/Last Update: 2013-04-29