[1]刘虎,陈育如,唐 刚,等.豚草中绿原酸的测定及提取剩余物的生物转化[J].南京师范大学学报(工程技术版),2009,09(04):087-92.
 Liu Hu,Chen Yuru,Tang Gang,et al.Determination of the Content of Chlorogenic Acid in Ambrosia artemisiifolia L. and Bioconversion of Extraction Residue[J].Journal of Nanjing Normal University(Engineering and Technology),2009,09(04):087-92.
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豚草中绿原酸的测定及提取剩余物的生物转化
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南京师范大学学报(工程技术版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
09卷
期数:
2009年04期
页码:
087-92
栏目:
出版日期:
2009-12-30

文章信息/Info

Title:
Determination of the Content of Chlorogenic Acid in Ambrosia artemisiifolia L. and Bioconversion of Extraction Residue
作者:
刘虎1 陈育如1 唐 刚1 刘军利2
1. 南京师范大学生命科学学院, 江苏南京210046;
2. 中国林业科学研究院林产化学工业研究所国家林业局林产化学工程重点实验室, 江苏南京210042
Author(s):
Liu Hu1Chen Yuru1Tang Gang1Liu Junli2
1.School of Life Science,Nanjing Normal University,Nanjing 210046,China;2.Key Laboratory on Forest Chemical Engineering of SFA,Institute of Chemical Industry of Forest Products of CAF,Nanjing 210042,China
关键词:
豚草 绿原酸 超声波 提取 生物转化
Keywords:
Am bro sia artem is iifo lia L. chlorog en ic ac id u ltrason icw ave ex trac tion bioconversion
分类号:
S451
摘要:
采用超声波法辅助提取、HPLC 测定的方法, 研究了豚草不同部位绿原酸的含量. 并与烟草、金银花和几种南京郊区常见杂草的绿原酸含量进行了比较, 将提取剩余物用间型脉孢菌进行了生物转化. 实验结果表明, 豚草各部位的绿原酸含量以叶最多, 为1. 160m g/ g; 花和茎次之, 分别为0. 559 和0. 494mg / g; 豚草叶绿原酸以7月份含量最高. 在50℃ 时用超声波辅助提取, 作用时间以20m in为宜. 用间型脉孢菌转化提取剩余物, 物料经生物转化后, 富含纤维素酶、蛋白酶和类胡萝卜素, 其中纤维素酶中CMC 酶活性为5 503. 9U / g, 蛋白酶活性为7 926.4U /g, 类胡萝卜素含量为126.6 μg / g. 提取酶和类胡萝卜素后的物料可作为生产有机肥的原料, 使物料得到全部利用.
Abstract:
The content o f ch lo rogenic ac id in d ifferen t parts ofAm bro sia ar tem isiifolia L. w as stud ied byHPLC analysis m ethod and u ltrasonic-assisted extraction. Chlorogen ic acid contents ofAm brosia ar tem isiifolia L. , N ico tiana tabacum, Lonicera japonica Thunb. and seve ra l comm on w eed o fN an jing w ere compared. The ex traction residues w ere trea ted by N eurosp ora intermed ia. The resu lts show ed that the contents of chlorogenic acid in d ifferent parts o fAm brosia artem isiifo- lia L. w ere d ifferen t. Leaves were 1.160m g /g, flow ers and stem s w ere 0.559 mg /g and 0. 494 mg /g respective ly. The ch lorogen ic ac id content o fAm bro sia artem isiifolia L. leaf is the h ighest in Ju ly. When ex tracted by ultrasound-assisted m ethod at 50℃ , the proper extrac tion tim e was 20 m in. After b ioconversion byN eurospora in term edia, the extraction res idues conta in caro teno id and abundance o f enzymes such as cellulase and pro tease, the y ields of ca rotenoid, as high as 126. 6 μ g /g, enzym e activ ity of CMCase and Pro teasew ere 5 503.9U /g and 7 926.4U /g, respectively. After the en- zym e and caro teno id w ere ex tracted from ferm enta tion products, the res iduew as entire ly utilized as o rganism fertilizer to- tally.

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备注/Memo

备注/Memo:
基金项目: 江苏省高技术研究计划( BG2007049)资助项目.
通讯联系人: 陈育如, 博士, 副教授, 研究方向: 生物化工. E-m ail:chenyu ru@ n jnu. edu. cn
更新日期/Last Update: 2013-04-23