[1]唐 刚,陈育如,刘友芬.间型脉孢菌产类胡萝卜素研究[J].南京师范大学学报(工程技术版),2009,09(01):073-77.
　Tang Gang,Chen Yuru,et al.Study on Carotenoids Production by Neurospora intermedia[J].Journal of Nanjing Normal University(Engineering and Technology),2009,09(01):073-77.
点击复制

间型脉孢菌产类胡萝卜素研究
分享到：

南京师范大学学报（工程技术版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
09卷
期数:
2009年01期
页码:
073-77
栏目:
出版日期:
2009-03-30

文章信息/Info

Title:
Study on Carotenoids Production by Neurospora intermedia
作者:
唐 刚1;  2;  陈育如1;  2;  刘友芬1;  2
1. 南京师范大学生命科学学院, 江苏南京210046;
2. 江苏省生物多样性与生物技术重点实验室, 江苏南京210046
Author(s):
Tang Gang1; 2; Chen Yuru1; 2; Liu Youfen1; 2
1.School of Life Science,Nanjing Normal University,Nanjing 210046,China;2.Jiangsu Key Lab for Biodiversity and Biotechnology,Nanjing 210046,China
关键词:
间型脉孢菌;  类胡萝卜素;  生物质;  添加物
Keywords:
N eurosp ora interm edia;  caro teno ids;  b iom ass;  aux iliary
分类号:
Q93
摘要:
筛选了一株产类胡萝卜素的间型脉孢菌(Neurospora intermedia),研究了该菌发酵过程中培养条件、添加物和光照条件对类胡萝卜素产量的影响.实验结果表明,间型脉孢菌产类胡萝卜素的适宜温度为30℃;当培养基中添加了1.5 mg/L没食子酸和0.05 g/L植酸钙后,所培养菌丝中类胡萝卜素含量分别可达46.44和54.03(μg/g干菌体),比对照产量分别提高了100%和133%(以干菌体计);增加光照对菌体产类胡萝卜素也有明显的促进作用,与对照相比,类胡萝卜素产量提高了73.7%.间型脉孢菌孢子中类胡萝卜素含量明显高于菌丝含量,达145.5μg/g孢子.固体培养时产孢量大,因此物料中类胡萝卜素产量可达81.94μg/g干曲.
Abstract:
The wo rk screened a strain o fN eurosp ora interm ed ia tha t y ie ld caroteno ids. The effect factors on output of caro teno ids such as culture cond itions, spec ies add itives and the illum ina tion fo rN. interm ed ia in the ferm enta tion pro cess w ere studied. The results show ed that optim um temperature o f produc ing caro teno ids w as 30e , when added 1.5 mg /L ga llic acid and 0.05 g /L phy tin respective ly into the culture med ium, the caro teno ids content of myce lium w as up to 46.44 and 54. 03 (μ g /g dry cell), the caro teno ids y ield increased by 100% and 133% ( dry ce ll) than the contro l respectively. Illum ination had an obv ious effect on prom oting ou tput o f caro te no ids, com pared w ith the contro,l the caro teno ids production increased by 73.7%. The caro teno ids content in spo res o fN. interm ed ia is as h igh as 145.5μ g /g( dry spore), sign ificantly h ighe r than that of my ce lium content. Spore production capac ity w as larger in cu ltu re so lid, so the ca rotenoids con tent o fm a terials w as 81.94μ g / g dry koji.

参考文献/References:

[ 1] Br itton G, L iaaen- Jensen S, PfanderH. Caroteno ids H andbook( V II) [M ] . B ase:l Boston B irkhause rVer lag, 2004.
[ 2] M a rtaM C, Gu ille rmo SM. E ffec t o f illum ination and ch lo rophy lls on stab ility of tom ato ca ro teno ids[ J]. Food Chem istry,
2008, 107( 4): 1 365-1 370.
[ 3] Steenbo ck H, W h ite co rn V S. Ye llow corn and a probable relation betw een the fat- so lub le v itam ine and ye llow p lant pigm ents
[ J] . Science, 1919, 50: 352-353.
[ 4] Pa rk P K, K im E Y, Chu K H. Chem ical disruption of yeast cells for the iso lation of caroteno id pigm ents[ J]. Separa tion and
Pur ification Techno logy, 2007, 53( 2): 148-152.
[ 5] Packo P L, M arcel J, R ic C H De Vos, e t a.l Exploring and explo iting caro teno id accum ulation in Duna lie lla sa lina for ce l-l
factory applica tions[ J]. Trends in B io techno logy, 2008, 26( 11): 631-638.
[ 6] H u C C, L in J T, Lu F J, et a.l Dete rm ina tion of caroteno ids inDunaliella salina cultivated in Ta iw an and antiox idan t capac ity
o f the a lga l caro teno id ex tract[ J]. Food Chem istry, 2008, 109( 2) : 439-446.
[ 7] M a r?a D Q R, RosaM R V, Santiago T M, et a.l Light induction o f the caro teno id b io syn thesis pathw ay in B lakeslea trispora
[ J] . Fungal Gene tics and B io logy, 2005, 42( 2): 141-153.
[ 8] 杨先泉, 赵勤, 傅体华. 脉孢霉两对基因顺序四分子分析[ J] . 遗传, 2008, 30( 6): 801-806.
Yang X ianquan, Zhao Q in, Fu T ihua. O rdered tetrad ana ly sis o f two genes inN eurospora[ J] . H ereditas, 2008, 30( 6) : 801-
806. ( in Ch inese)
[ 9] M a rko Za loka r. Studies on b iosynthes is o f caroteno ids in N eurosp ora crassa [ J] . Arch ives of B iochem istry and B iophys ics,
1954, 50( 1): 71-80.
[ 10] 王岁楼. 红酵母产生类胡萝卜素的研究[ J]. 中国粮油学报, 2001, 16( 5) : 43-46.
W ang S L. S tudy on the conditions o f caroteno id ferm enta tion by R hodotorula [ J]. Chinese C erea ls and O ils A ssoc ia tion,
2001, 16( 5): 43-46. ( in Ch inese)
[ 11] 左亚军. 环境因素和培养条件对类胡萝卜素表达的影响[ J]. 上海化工, 2007, 32( 2): 22-26.
Zuo Y J. E ffect o f d ifferent env ironm enta l and cu ltura l stim ulants on the production of caro teno ids[ J]. Shangha iChem ica l Industry,
2007, 32( 2): 22-26. ( in Ch inese)
[ 12] 张晓蓉, 马文丽, 杨素萍. 产氢红杆菌类胡萝卜素代谢研究[ J] . 山西大学学报: 自然科学版, 2006, 29( 2): 201-204.
Zhang X R, M aW L, Yang S P. Ca rotenoid m etabo lism of hydrog en-produc ing Rhodobacter sp. [ J]. Journa l o f Shanx i Un-i
versity: Natural Science Ed ition, 2006, 29( 2): 201-204. ( in Ch inese)

相似文献/References:

[1]戴亦军,袁生,秦怀兰.深红酵母产生的类胡萝卜素对蛋黄着色的研究[J].南京师范大学学报(工程技术版),2004,04(04):055.
　DAI Yijun,YUAN Sheng,QIN Huailan.Yolk Pigmentation of Carotenoid Produced from Rhodotorula rubra[J].Journal of Nanjing Normal University(Engineering and Technology),2004,04(01):055.

备注/Memo

备注/Memo:
基金项目: 江苏省高技术研究计划( BG2007049)资助项目.
通讯联系人: 陈育如, 博士, 副教授, 研究方向: 生物化工与应用微生物. E-m ail:chenyu ru@ n jnu. edu. cn
更新日期/Last Update: 2013-04-23